菲立磁标记的大鼠骨髓基质干细胞在同种异体移植鼠肝脏中的追踪观察

Tracking of allogenically grafted rat bone marrow stem cells labeled with Feridex in rat liver

目的:研究菲立磁标记的大鼠骨髓基质干细胞(BMSCs)在同种异体移植鼠肝脏中的追踪方法.方法:采用菲立磁(Feridex)对分离培养的BMSCs进行标记,Prussianblue染色和电镜观察对标记后的BMSCs进行鉴定;取鉴定后的BMSCs2mL(1×109/L)分别注射到SD异体大鼠的门静脉、肝局部实质、尾静脉内;采用磁共振成像(MRI)技术(SE-T2WI,FSE-T2WI,GRE-T2WI序列)分别对移植前6h,移植后6h,1,3和5wk时的大鼠肝脏进行扫描.结果:Prussianblue染色证实Feridex标记BMSCs阳性率>90%,透射电镜见铁颗粒位于BMSCs胞质中;MRI扫描SE-T2WI序列成像显示:各移植组在移植前肝脏均未见异常低信号区;门静脉组大鼠移植后6h在肝门部可见异常的低信号改变,1,3wk低信号改变范围逐渐扩大,第5周时低信号改变消失;肝内注射组大鼠移植后6h在肝脏局部注入区域可见异常的低信号改变,1,3,5wk低信号改变区域逐渐扩大.尾静脉组在移植后各时间点均未见到异常低信号改变.结论:Feridex标记的大鼠BM-SCs在同种异体移植鼠肝脏中可用MRI进行有效的追踪.

AIM： To study the method of tracking of allogenically grafted rat bone marrow stem cells （ BMSCs ） labeled with Feridex in rat liver. METHODS..Feridex was used to label isolated BMSCs. Labeled BMSCs were examined by Prussian blue staining and electron microscopy. Successfully labeled BMSCs （ 2.0 mL, 1 × 10^9/L） were respectively transplanted through portal vein, local liver parenchyma and caudal vein of the SD rats. Six hours before transplantation and 6 h, 1,3, and 5 weeks after transplantation, magnetic resonance imaging （MRI） examination （the sequences used were SE-T2WI, FSE-T2WI, GRE-T2WI） was conducted on rat livers. RESULTS： Prussian blue staining confirmed the labeling efficiency was above 90%, and transmission electron microscopy showed numerous iron particles in the cytoplasm of Feridex-labeled BMSCs. MRI （ SE-T2 WI） showed ： no remarkable low signal change was found in the liver images before transplantation of all 3 groups. In the porta hepatis of portal vein transplantation group remarkable low signal changes were seen 6 h after transplantation and the extent gradually expanded 1 and 3 weeks after transplantation, while the low signal change could not be seen 5 weeks after transplantation. In the section where the cells were injected in of liver topical transplantation group remarkable low signal changes were seen 6 h after transplantation and the extent gradually expanded 1, 3 and 5 weeks after transplantation. No remarkable low signal change was found in the liver images of caudal vein transplantation group at all time points after transplantation. CONCLUSION： Allogenically grafted Feridex-labeled BMSCs can be tracked effectively in rat liver by MRI.