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血清双向电泳样品制备技术的优化及应用 被引量：2

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作者 赵亮 李欣 李雪华 丁彦青
机构地区 南方医科大学南方医院病理科 武警医学院病理学教研室
出处 《第四军医大学学报》 北大核心 2006年第21期F0003-F0003,共1页 Journal of the Fourth Military Medical University
基金 广东省科技重大专项(2003A308401) 国家重点基础研究发展规划973项目2004年子课题(2001CB510207)
关键词 人血清蛋白质 样品制备 双向电泳 蛋白质组
分类号 R730.3 [医药卫生—肿瘤]
  • 相关文献

参考文献4

  • 1李雪华,李欣,徐守军,丁彦青.血清蛋白质组学研究中白蛋白和IgG去除方法的建立与评价[J].第一军医大学学报,2004,24(11):1233-1235. 被引量：11
  • 2Herbert B.Advances in protein solubilisation for two-dimensional electrophoresis[J].Electrophoresis,1999,20(4-5):660-663.
  • 3Joo WA,Lee DY,Kim CW.Development of an effective sample preparation method for the proteome analysis of body fluids using 2-D gel electrophoresis[J].Biosci Biotechnol Biochem,2003,67(7):1574-1577.
  • 4Chen JH,Chang YW,Yao CW,et al.Plasma proteome of severe acute respiratory syndrome analyzed by two-dimensional gel electrophoresis and mass spectrometry[J].Proc Natl Acad Sci U S A,2004,101(49):17039-17044.

二级参考文献15

  • 1Steel LF, Shumpert D, Trotter M, et al. A strategy for the comparative analysis of serum proteom es for the discovery of biomarkers for hepatocellular carcinoma [ J ]. Proteomics, 2003, 3(5): 601-9.
  • 2Rai AJ, Zhang Z, Rosenzweig J, et al. Proteomic approaches to tumor marker discovery[J]. Arch Pathol Lab Med, 2002, 126(12):1518-26.
  • 3Wang YY, Cheng P, Chan DW. A simple affinity spin tube filter method for removing high-abundant common proteins or enriching low-abundant biomarkers for serum proteomic analysis [J]. Proteomics, 2003, 3(3): 243-8.
  • 4Rui Z, Guo JJ, Yuan-PT, et al. Use of serological proteomic methods to find biomarkers associated with breast cancer [J]. Proteomics,2003, 3(4): 433-9.
  • 5Li J, Zhang Z, Rosenzweig J, et al. Proteomics and bioinformatics approaches for identification of serum biomarkers to detect breast cancer [ J ]. Clin Chem, 2002, 48(8): 1296-304.
  • 6Gorg A, Postel W, Gunther S. The current state of two-dimensional electrophoresis with immobilized pH gradients [ J ]. Electrophoresis,2000, 21(6): 1037-53.
  • 7Mortz E, Krogh TN, Vorum H, et al. Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis [ J ].Proteomics,2001, 1(11): 1359-63.
  • 8Adkins JN, Varnum SM, Auberry KJ, et al. Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry [J]. Mol Cell Proteomics, 2002, 1 (12):947-55.
  • 9Ma PC, Blaszkowsky L, Bharti A, et al. Circulating tumor cells and serum tumor biomarkers in small cell lung cancer [J ]. Anticancer Res, 2003, 23(1A): 49-62.
  • 10Anderson NL, Anderson NG. The human plasma proteome: history,character, and diagnostic prospects [J]. Mol Cell Proteomics, 2002,1(11): 845-67.

共引文献10

同被引文献18

  • 1王征,阮幼冰,官阳.肝细胞癌患者血清蛋白质组成分的双向凝胶电泳-飞行时间质谱分析[J].中华病理学杂志,2003,32(4):333-336. 被引量：17
  • 2刘希成,梁恒,田真,张霖,陈阳.血清蛋白质组样品处理方法的比较分析[J].第四军医大学学报,2007,28(7):640-640. 被引量：2
  • 3Fleck RA, Romero-Steiner S, Nahm MH. Use of HL-60 cell line to measure opsonic capacity of pneumococcal antibodies [ J ]. Clin Diagn Lab Immunol, 2005,12 ( 1 ) : 19 - 27.
  • 4Kaiser T, Kamal H, Rank A, et al. Proteomics applied to clinical follow up of patients after allogeneic hematopoietic stem cell transplantation [J]. Blood, 2004,104 ( 2 ) : 340 - 349.
  • 5Saravanan RS, Rose JK. A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues[ J], Proteomics, 2004,4 (9) :2522 - 2532.
  • 6Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976,72( 1 -2) :248 -254.
  • 7Grebenova D, Halada P, StulikJ, et al. Protein changes in HI-60 leukemia ceils associated with 5-Aminolevulinic acid-based photodynamic therapy. Early Effects on Endoplasmie Reticulum Chaperones [ J]. Photochem Photobiol,2000,72( 1 ) :16 -22.
  • 8Steel LF,Shumpert D,Trotter M,et al.A strategy for the comparative analysis of serum proteomes for the discovery of biomarkers for hepatocellular carcinoma.Proteomics,2003,3 (5):601-609.
  • 9Lu Z,Hu L,Evers S,et al.Differential expression profiling of human pancreatic adenocarcinoma and healthy pancreatic tissue.Proteomics,2004,4(12):3975-3988.
  • 10Goncalves A,Esterni B,Bertucci F,et al.Postoperative serum proteomic profiles may predict metastatic relapse in high-risk primary breast cancer patients receiving adjuvant chemotherapy.Oncogene,2006,25(7):981-989.

引证文献2

二级引证文献5

第四军医大学学报
2006年 第21期

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