摘要
亲水作用色谱是采用极性固定相、高含量极性有机溶剂-水相缓冲液为流动相的一种分离技术,它能有效地保留反相色谱中保留弱或不保留的强极性碱性药物。本文综述了以未键合硅胶为固定相的亲水作用色谱分离碱性药物的机理、特点及其应用的最新进展。
Hydrophilic interaction chromatography （HILIC） is a convenient method for separating basic drugs and their metabolites which have poor retention on reversed-phase column by passing highly polar organic-aqueous buffer mobile phase across a polar stationary phase. The mechanism and characterization of HILIC using silica column/reversedphase elution system are described. In addition, the recent progress of its applications in the separation of basic pharmaceuticals is reviewed.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2006年第11期1508-1513,共6页
Progress in Chemistry
关键词
亲水作用色谱
碱性药物
分离
hydrophilic interaction chromatography
basic drugs
separation
