胃蛋白酶诱导碳酸钙结晶的研究

The Study of Pepsin Inducing the Precipitation of Calcium Carbonate

钙盐通常出现在各种类型的胆结石中。基质蛋白质被公认为生物矿化的重要因素。许多研究表明蛋白质在胆结石的形成过程中起到了非常重要的作用,为此选用了胃蛋白酶作为添加剂在仿生物环境的条件下诱导碳酸钙的生成,更有利于研究胆结石中碳酸钙形成的可能机理。FT-IR,XRD和SEM测试结果表明,不同浓度的胃蛋白酶诱导了不同形貌的碳酸钙的生成,但这些结晶都属于方解石型碳酸钙。

Calcium salts are often present in the centre of all types of gallstones. Matrix proteins are known to be essential for biomineralization and may also be important in the formation and growth of gallstones. In the present work we employed pepsin in order to assess its capability to induce the formation of CaCO3 in biological circumstances in vitro. The appearance and morphology of CaCO3 crystals obtained from systems with and without pepsin were observed by FT-IR, XRD and SEM. The results showed that the pepsin played an important role in the regulation of precipitation of calcium salts during the formation of gallstones. The result confirmed they were all calcite regardless of the different morphology.