灵芝液体发酵产漆酶最佳培养基和培养条件研究

Optimum culture media and liquid conditions in shaking flasks for laccase production by Ganoderma lucidum

目的研究灵芝液体发酵产漆酶的最佳培养基和培养条件。方法以灵芝S3号菌株为试验材料,以灵芝漆酶活性为衡量指标,通过正交试验分别对灵芝液体发酵培养基及培养条件进行优化筛选。结果筛出最佳培养基组成为葡萄糖30g/L、棉花0.2%、磷酸氢二铵0.66g/L、干酪素0.5%、聚山梨酯-800.15%;优化培养条件为初始pH5.5、装液量75mL、接种量12.5%、菌龄5×24h、培养时间9×24h。结论优化培养基及培养条件后,发酵液中灵芝漆酶活性显著提高。

Objective To study the optimum culture media and liquid conditions in shaking flasks for laccase production by Ganoderma lucidum. Methods Taking S3 stain of G. lucidum as test materials and laccase activity of G. lucidum as measurement index to optimize the culture media and liquid conditions through orthogonal test. Results The optimum culture components of media were ： Glucose 30 g/L, cot ton 0.2%, （NH4）2HPO4 0.66 g/L, casein 0.5 %, Tween-80 0. 15 mL; the optimum conditions were： ini tial pH value of medium was 5.5, 75 mL medium was in 250 mL-flask, inoculation was 12. 5%, mycelium age was 5 × 24 h for 9 ×24 h culture. Conclusion Laccase activity of G. lucidum has been improved remarkably in the optimum culture media and liquid conditions.