降解甲基对硫磷的节杆菌(Arthrobacter sp.)L4菌株的分离和降解特性研究

Isolation,degradation and characterization of methylparathion degradative strain L4

从生产甲基对硫磷的山东华阳农药厂污水曝气池中,分离到一株能以甲基对硫磷及其降解中间产物对硝基苯酚为唯一碳源生长的细菌L4菌株.经16SrRNA基因序列分析,该菌株被鉴定为节杆菌属(Arthrobactersp.).用气相色谱法和分光光度法分析L4菌株的降解性能,结果表明,L4在5h内对50mg.L-1的甲基对硫磷和对硝基苯酚的降解率分别为85%和98%,对其它有机磷农药也有良好的降解效果.L4的最适培养条件为pH值7、30℃、接种量30%.

From waste water of Shandong Huayang Pesticide Chemical Industry Group Co. , Ltd, a strain L4 was isolated, which could degrade methylparathion and p-nitrophenol, and used them as the sole carbon source . Through physiological and biochemical tests and 16S rRNA genetical analysis, L4, was identified as Arthrobacter sp.. The degradative ability to pesticides of L4 was determined by gas chromatography （GC） and spectrophotometer, the degradative rate for methylparathion and p-nitrophenol at 50mg· L^-1 was 85% and 98% respectively within 5 hours at 30℃ , pH 7 ,with inoculating quantity at 30%. Moreover, L4 could degrade other organophosphate pesticides quickly.