用不同检测物测定乙型肝炎病毒核酸含量方法的研究

The Research on the Method of Measuring the Content of Nucleic Acid of HepatitisⅡ Virus from Different Ttesting Samples

目的探讨乙肝患者不同的检测物(同时取全血与血清)检测其HBVDNA含量及临床意义。方法用荧光定量PCR法检测全血和血清中HBVDNA含量。结果对362例乙型肝炎患者的全血和相应血清HBVDNA定量检测,结果发现:1.全血HBVDNA检出率为97.0%,血清HBVDNA检出率为89.8%,两者检出率有显著差异(P>0.05);.2.在HBV血清标志:HBsAg(+)HBeAg(+)HbcAb(+);HBsAg(+)HbeAb(+)HbcAb(+);HBsAg(+)HbcAb(+);HBsAg(+)各组中,其全血和血清的HBVDNA检出率和含量按其排序均逐渐下降;但血清比全血下降得快;3.在不同类型乙肝患者中,除88例急性肝炎全血和血清HBVDNA检出含量相近外,其余的全血HBVDNA含量均高于其血清含量。结论除急性肝炎外,全血标本检测HBVDNA含量高于其血清的HBVDNA含量。全血检测结果更能反映其病毒的实际含量,在临床治疗监测中更有指导意义。

Objective Clinical analysis the content of HBV DNA in different samples （whole blood and serum） from the hepatitis patients. Methods Testing the HBV DNA content in samples with whole blood and serum respectively by Quantified Fluorescence PCR. Results 362 samples with whole blood and corresponding serum from hepatitis Ⅱ patients have been quantified examined respectively, and we discovered： 1. the HBV DNA had been observed in 97.0% of overall whole blood samples, and the effective rate for serum samples was only 89.8%. There is significant difference between both above （P〉0.05）. 2. in the following groups with which different HBV： HBsAg（＋） HBeAg（＋） HbcAb（＋）; HBsAg（＋） HbeAb（＋） HbcAb （＋）; HBsAg （＋）HbcAb （＋）; HBsAg （＋）, both the values of the effective rate and contents of HBV DNA were reducing in terms of such sequence in either whole blood samples or samples with serum, but those values from serum samples were dropping more severe than that in samples with whole blood. 3. comparing the samples from various hepatitis patients, the contents of HBV DNA in all whole blood samples are higher than that in serum apart from 88 samples from acute hepatitis patients which those two values were very closed. Conclusion apart from acute hepatitis, the content of HBV DNA in whole blood samples is more than that in samples with serum; therefore, the whole blood test is more important in the clinical treatment due to its capability to reflect the true value of the content of virus.