不同基因型油菜锌效率机理Ⅰ——耐低锌油菜基因型苗期筛选指标研究

Study on Zn Efficiency of Different Genotypes in Rape —— An approach to the screening biomarkers for rape seedlings tolerant to low Zn

采用营养液培养试验比较了低锌营养下芥菜、白菜、甘蓝型3个油菜(BrassicanapusL.)品种幼苗的生物量,抗氧化酶活性,根系活力,叶绿素含量及对锌的吸收、利用差异。试验结果表明,3个油菜品种由于基因型不同,在耐低锌营养能力方面存在显著性差异。在低锌时(10-6mol/LZn2+),幼苗植株地上部干重以芥菜型>甘蓝型>白菜型,地上部干重分别较无锌对照处理增加17.8%(芥菜型)、9.9%(白菜型)、3.3%(甘蓝型)。芥菜型叶CAT、POD、SOD活性,叶绿素含量及根系活力也明显高于甘蓝型和白菜型油菜。锌积累量及锌利用率也以芥菜型远远大于其它2个品种,锌素利用效率分别为12.3%(芥菜型),8.0%(白菜型),4.9%(甘蓝型)。苗期油菜叶CAT、POD、SOD等抗氧化酶活性、叶绿素含量及根系活力可以作为筛选耐低锌营养油菜基因型的评价指标之一。

Investigations were tioxidant enzymes, root system carried out to evaluate the influence of low Zn tolerant on plant growth, anvigor, chlorophyll and absorption and utilization of Zn in three varieties of rape （Brassica napus L.） by solution experiment. The results showed that significant differences of tolerant were recorded in response to low Zn^2＋ level （10-6mol/L Zn^2＋） among the rape seedlings due to different genotypes. Under low Zn treatments, shoot dry weight of seedlings was in the order of Brassica juncea〉 Brassica napus 〉 Brassica campestris, and increased by 17.8% （ Brassica junce）, 9.9% （Brassica campestri）, 3.3% （ Brassica napus） compared to the control. The higher activities of CAT, POD, SOD, concentration of chlorophyll, and root tolerance were found in Brassica juncea, and its Zn uptake and Zn utilization efficiency were higher than Brassica napus and Brassica campestris. Zn utilization efficiency of be act as the biomarkers for screening rape genotypes tolerant to low Zn.