广州市某野生动物市场从业人员携带SARS冠状病毒抗体状况调查

Study on the dynamic prevalence of serum antibody against severe acute respiratory syndrome coronavirus in employees from wild animal market in Guangzhou

目的了解广州市新源野生动物市场从业人员严重急性呼吸综合征(SARS)抗体产生情况及其暴露果子狸的关系。方法对野生动物市场从业人员中的志愿者进行SARS病毒血清抗体监测,对监测结果结合果子狸在市场的存在情况进行分析。结果2003年5和12月、2004年1和7月、2005年6月,共5次分别对328、238、135、139和53名市场从业人员开展调查,SARS病毒IgG抗体检出率依次为25．61%、13．03%、12．59%、5．04%和9．43%,没有检测到SARS病毒IgM抗体。在接受2次以上随访调查的129人中,97人的血清抗体一直为阴性,18人一直为阳性；有13人从阳性转为阴性,有1人从阴性转为阳性。在清除果子狸后进入市场的工作人员,SARS病毒IgG抗体检测全部阴性。结论广州市新源野生动物市场从业人员SARS病毒抗体检出率和市场内果子狸的存在情况密切相关。

Objective To investigate the dynamic trend of specific antibody against severe acute respiratory syndrome（SARS）-CoV in serum collected at various periods among employees in Guangzhou Xinyuan animal market. Methods Volunteers from employees of the animal market were recruited and their serum specific antibody against SARS-CoV were determined by enzyme linked immunesorbent assay （ELISA） method. Results Positive SARS-CoV specific IgG antibody was found 25.61% （ n = 328）, 13.03 % （ n = 238）, 12.59 % （ n = 135）, 5.04 % （ n = 139） and 9.43 % （ n = 53） among volunteers, which were sampled in May 2003, Dec. 2003, Jan. 2004, July 2004 and June 2005 respectively. No specific IgM antibody was found in all of those samples. Among 129 samples which were tested twice or more, 97 were all negative, 18 all positive, 13 changed from positive to negative but only one sample from negative to positive. When the volunteers were divided by the duration of their working experiences as short-term or long-term, those who had worked at animal market for less than or more then 6 months when being tested, the positive rate for long-term employees were relatively constant, however, all of the persons employed after January 2004, when the palm civets and raccoon dogs were culled from the market, were tested negative. Conclusion The prevalence of specific antibody against SARS-CoV in employees of the animal market were somehow related with the presence or absence of palm civet. No serum was tested positive for persons who were employed after palm civets and raccoon dogs were culled from market. This data indicated that the SARS-CoV might have been from the palm civets and raccoon dog, and the animal market seemed to serve as one of the sources of infection.