烟草种质资源遗传多样性及亲缘关系的AFLP分析

Studies on Genetic Diversity and Relationship in Tobacco Germplasms by AFLP Analysis

【目的】揭示烟草种质资源的遗传多样性及亲缘关系。【方法】选用4对AFLP选择性扩增引物(EcoRI+3/MseI+3)对48份烟草材料的基因组DNA进行选择性扩增。【结果】共获得321条扩增带,其中174条具有多态性,平均多态检出率为54.2%。对AFLP扩增结果采用UPGMA法进行聚类分析,可以将48份烟草资源分为两大类群,即黄花烟草群和普通烟草群,普通烟草可进一步分为4组;48份材料的遗传距离变幅在1.41～11.0之间。【结论】AFLP标记技术能较好地从分子水平揭示中国烟草种质资源的遗传背景、亲缘关系及演化规律。

[ Objective ] This study was carded out in order to analyze genetic diversity and relationship in tobacco germplasms. [Method] Genomic DNA of 48 tobacco materials were amplified by 4 AFLP selective primers（EcoRI＋3/MseI＋3）. [Result] A total of 321 AFLP bands were obtained including 174 polymorphic ones. The percentage of polymorphic bands is 54.2%.UPGMA cluster analysis based on AFLP molecular markers data divided 48 varieties into two groups： N.tobacum L group and N.rustica L.group. N.tobacum L. group could be classified into four sub groups. The genetic distance of 48 cultivars was between 1.41 and 11.0. [ Conclusion] The results showed that AFLP approach is efficient in generating more accurate information on genetic background, relationship and evolution of tobacco germplasms.