摘要
对于高效液相色谱而言,温度是一个一直被忽视而又非常重要的因素。升高色谱分析的柱温,不仅可以加快分析速度,而且还可以提高分离效率、改善峰形、调节选择性、减少有机溶剂的使用,是一个非常有效而又十分经济适用的方法,与其它调节分离效果的方法相比,具有其独特的优势。文中综述了温度对高效液相色谱分离特征的影响,介绍了温度作为一个便捷的调节参数在高效液相色谱中的应用前景。
Temperature is an important factor of HPLC performance. Because of the elevated temperature, viscosity of the mobile phase decreased, backpressure over the column reduced, the speed of mobile phase can be improved, and shorten the analytical time and adjust the selectivity. At the same time, under the higher temperature, the use of organic solvent can be decreased, Elevated the analytical temperature is an efficient and economic method for high performance chromatography. The influence of temperature on Separation Behaves of HPLC is reviewed in this paper.
出处
《现代科学仪器》
2006年第5期74-78,共5页
Modern Scientific Instruments
关键词
温度
高效液相色谱
综述
Temperature
high performance liquid chromatography
review
