摘要
将CaMV35S启动子驱动的内质网小分子热激蛋白基因导入番茄,比较转基因、未转基因和转空载体番茄的抗冷能力。冷胁迫下,同对照相比,转基因番茄的冷害症状轻,叶绿素含量的损失少,体内积累的丙二醛(MDA)含量少,电解质外渗程度低,具有较高的净光合速率和最大光化学效率,并易于恢复由低温所引起的光抑制,表明转基因番茄具有较强的冷胁迫耐性,说明内质网小分子热激蛋白在植物抗冷过程中发挥重要作用。
The full-length endoplasmic reticulum small heat shock protein （ER-sHSP） cDNA using CaMV 35S promoter was transformed into the genome of tomato plants. The chilling tolerance of transgenic tomato plants, non-transgenic tomato plants and pROK Ⅱ-transformed tomato plants were studied. Under chilling stress treatment, compared with non-transgenic and pROK Ⅱ-transformed tomato plants, transgenic tomato plants exhibited slighter cold-injured symptoms, suffered less destruction of chlorophyll and electrolyte leakage. The content of MDA of transgenic tomato plants was lower than that of non-transgenic and pROK Ⅱ -transformed tomato plants, and transgenic tomato plants could keep up higher value of net photosynthetic rate, Fv/ Fm and could recover quickly from chilling-induced photosynthetic inhibition. The results showed that ER- sHSP played a key role in enhancing the chilling-resistance ability of plants.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第5期989-994,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30270132)
关键词
番茄
内质网
小分子热激蛋白
转基因
冷胁迫
Tomato
Endoplasmic reticulum
Small heat shock protein
Transgenic
Chilling stress
