摘要
以‘土佐文旦’柚成树为材料,探讨了秋水仙素诱导2n配子的方法,研究了秋水仙素浓度和处理时期对获得三倍体的影响。结果表明:开花前1周用0·25%的秋水仙素处理花蕾,可诱导2n配子自交亲和。经对发芽存活的自交后代植株的叶片细胞核DNA量检测和细胞学鉴定,确定25%的自交后代植株为自交三倍体。通过秋水仙素诱导文旦2n配子是获得自交三倍体的有效途径。
Tosa-Buntan pummelo [ Citrus grand/s （ L. ） Osb. ] was used to establish a method of 2n gametes induced by colchicine. The effects of colchicine concentration and its treatment period on the derived self-hybridized triploids were also analyzed. The results showed that 2n gametes with self-compatibility could be induced by treating the bud with 0. 25% colchicine at 1 week before flowering. 25% of the alive Fl plants were considered as the self-hybridized triploids after the measurement of leave cellular nucleus DNA content and cytological identification. Therefore, it is the effective method to obtain the self-hybridized triploids derived from 2n gametes induced by colchicines in pummelo.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第5期1045-1047,共3页
Acta Horticulturae Sinica
