梨矮化中间砧S_2、S_5和PDR_(54)的离体培养研究

Study on Tissue Culture in Vitro of Pear Dwarfing Interstocks S_2 , S_5 and PDR_(54)

以梨矮化中间砧S2、S5和PDR54试管苗为试材，探讨了培养基和植物生长调节剂对梨矮化中间砧试管苗增殖和生根的效应。其结果表明：MS培养基较QL、AS和WPM培养基更有利于中间砧茎的增殖，2—3mg·L^-1 6-BA、0．1—0．2mg·L^-1 IBA和1—2mg·L^-1 GA3组合能有效促进S2、S5和PDR54试管苗的增殖，其增殖倍数达3．71—5．83。综合各品种增殖倍数、茎高度以及茎质量等指标表明，MS＋3．0mg·L^-1 6-BA＋0．2mg·L^-1 IBA＋2．0mg·L^-1 GA3是S2的最佳增殖培养基；MS＋2．0mg·L^-1 6·BA＋0．1mg·L^-1 IBA＋1．0mg·L^-1 GA3是S5和PDR54增殖的最佳培养基。同时，S2、S5和PDR54增殖的试管苗在不同的生根条件下分别获得了67％、50％和86％的生根率。

The influencing factors of multiplication coefficient, regeneration quality and rooting for adventitious shoots of pear dwarfing interstocks S2, S5 and PDR54 were studied. The results showed that the regeneration ability of adventitious shoots was influenced by genotypes, media and plant growth regulators. MS medium was more suitable for the proliferation of S2, S5 and PDR54 than QL, WPM and AS media. The middie concentrations of 6-BA （2 - 3 mg · L^- 1 ） in combination with lower concentrations of IBA （0. 1 - 0. 2 mg · L^- 1 ） and GA3 （ 1 - 2 mg·L^- 1 ） could improve the proliferation of all the three interstocks, with proliferous index of shoots from 3.71 to 5.83. The best medium for S2 was MS ＋3.0 mg · L^-1 6-BA ＋0. 2mg · L^-1 IBA ＋2. 0 mg · L^-1 GA3, while MS ＋2. 0 mg ·L^-1 6-BA ＋0. 1 mg ·L^-1 IBA ＋ 1.0 mg · L^-1 GA3 was the optimal for S5 and PDR54 proliferation in comparison of the comprehensive index of shoot quality, numbers and lengths. The regenerated shoots were rooted with a rooting percentage of 67%, 50% and 86% respectively for S2, S5 and PDR54.