云南野生兰属主要种间亲缘关系的AFLP分析

Phylogenetics Analysis of the Chinese Orchids in Yunnan Province Using AFLP Technique

采用AFLP技术对云南省兰属的14个种和3个变种中的38份样本进行了亲缘关系分析。16对AFLP引物扩增的条带聚类结果表明,云南省国兰遗传多样性非常广泛,相同品种间的不同个体扩增出的条带模式相差很大。依据AFLP数据,在相似系数0·58的水平下,38个兰花样品分成3组:莲瓣兰、春剑、惠兰、寒兰分为第1组,建兰、豆瓣兰、春兰,墨兰、虎头兰、西藏虎头兰、大雪兰、碧玉兰、文山红柱兰、长叶兰和兔耳兰分为第2组,送春和蜜蜂兰分为第3组。

Thirty-eight collections of Chinese orchids in Yunnan Province, China, including 14 species and 3 varieties, were analyzed phylogenetically using AFLP technique. PCR amplification band pattern with 16 pairs of primers indicated that the Chinese orchids were very diverse, and individuals of the same species had different band patterns. Subgenus Jensoa appeared as a paraphyletic group. Subgenus Cyperorchis was not a monophyletic group instead formed an offshoot of subgenus Jensoa. At a similarity coefficient of 0. 58, the orchids could be clustered into 3 groups： Cybidium tortisepalum Wu et Chen, C. tortisepalum var. longibracteatum, C. faberi and C. kancan belonged to Group 1; C. ensifolium, C. goeringii var. serratum, C. goeringii, C. sinense, C. hookerianum, C. tracyanum, C. mastersii, C. lowianum, C. wenshanense, C. erythraeum and C. lancifolium to Group 2; C. cyperifolium var. szechuanicum and C. pumilum to Group 3.