E-选择素基因A561C(S128R)多态性与原发性高血压的关系研究

Relationship between E-selectin gene A561C polymorphism and essential hypertension

目的 探讨在中国人群中E-选择素基因A561C（S128R）多态性与原发性高血压的关系。方法 95例≤70岁的原发性高血压患者和101例年龄性别匹配的正常对照者，聚合酶链反应-限制性片段长度多态性方法检测E-选择素基因型．同时检测血脂、血糖、尿素、肌酐水平，ELISA测定血浆可溶性E-选择素水平。结果 我们发现3个基因型（AA，AC，CC），E-选择素基因型AA，AC，CC频率在原发性高血压组和对照组分别为78．9％、19％、2．1％和91．1％、7．9％、1．0％；等位基因A、C频率在原发性高血压组和对照组分别为88．4％、11．6％和95％、5％。其基因型频率和等位基因频率在原发性高血压组和对照组比较差异均有显著性（P（0．05）。基因型频率的相对风险分析表明AC基因型患原发性高血压的风险是AA基因型2．73倍（OR=2．73，95％CI=1．10～6．91）。结论 E-选择素基因A561C（S128R）多态性明显影响血压，C等位基因可能是原发性高血压的危险因素之一。

Objective To explore the relationship of the polymorphism of E-seleetin gene A561C and essential hypertension （EH） among Chinese people. Methods Genotypes of E-selectin were analyzed in 95 EH patients with age ≤70 and 101 normal controls people matched in age and gender by polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP）. Serum levels of lipid, glucose, urea and creatinine were measured by biochemical techniques. Plasma soluble E-selectin were measured by enzyme-linked immunosorbent assay（ELISA）. Results The frequency of E-selection genotypes AA, AC and CC in EH group were significantly higher than normal group （P 〈 0.05 ）. The frequencies of A and C allele in EH group were significantly lower than normal group （P 〈 0.05）. Relative risk analysis of genotypic frequency indicated that the risk of EH in AC genotype was 2.73 times higher than in AA genotype （ OR = 2.73,95 % CI = 1.10 - 6.91 ）. Conclusion E-selectin gene A561C polymorphism can significantly affect hypertension, C allele may be a risk factor of essential hypertension.