摘要
目的观察维生素D受体(VDR)、核心结合因子α1(Cbfα1)mRNA在大鼠成骨细胞(OB)中的表达,研究固本壮骨胶囊、金匮肾气丸、补肾益精方、知柏地黄丸四种补肾中药对大鼠成骨细胞VDR、Cbfα1mRNA表达的影响,探讨补肾中药促进骨形成的新机制。方法6月龄大鼠成骨细胞被分离,采用组织块翻转方法培养。通过倒置显微镜观察细胞形态,矿化结节染色对成骨细胞加以鉴定。采用RT-PCR方法检测成骨细胞VDR、Cbfα1mRNA表达。结果RT-PCR结果表明,在大鼠成骨细胞中,固本壮骨胶囊、金匮肾气丸、西药萌格旺上调VDR、Cbfα1mRNA表达;知柏地黄丸下调VDR、Cbfα1mRNA表达、补肾益精方对于VDR、Cbfα1mRNA表达的影响存在着差异。结论固本壮骨胶囊和金匮肾气丸上调成骨细胞VDR、Cbfα1mRNA表达,可促进骨形成;知柏地黄丸下调VDR、Cbfα1mRNA表达,有抑制或是降低骨形成的作用。
Objective ：To investigate the effects of reinforcing Kidney Chinese herbal medicine such as Guben-Zhuanggu （ 固本壮骨）, Jingui-Shenqi （ 金匮肾气 ）, Bushen- Yijing （补肾益精）, Zhibai-Dihuang （地黄） on expression of VDR, Cbfα1mRNA and explore the underlying mechanisms. Methods： Osteoblast of 6- month-age rats was isolated and cultured by the tissue-fragment-migrating-growth method. Biological characteris- tic was observed by phase-contrast microscope, mineralizing nodule staining technique and electromicroscope. The expression of VDR, Cbfα1 mRNA was detected by RT-PCR. Results：RT-PCR showed that Guben-Zhuanggu capsule, Jingui-Shenqi pill up-regulated the expression which was the same as Bone-one and Zhibai-Dihuang pill down-regulated the expression of VDR, Cbfα1mRNA in rat osteoblast, but the expression was different in Bushen-Yijing decoction. Conclusion：Guben-Zhuanggu capsule and Jingui-Shenqi pill promoted bone formation by up-regulating the expression of VDR, Cbfα1 mRNA but Zhibai-Dihuang pill inhibited the bone formation by down-regulating the expression.
出处
《中国骨伤》
CAS
2006年第11期659-661,共3页
China Journal of Orthopaedics and Traumatology
关键词
补肾药
成骨细胞
基因表达
Kidney reinforcing drugs
Osteoblasts
Gene expression
