壳寡糖与D-氨基葡萄糖盐酸盐体外对小鼠淋巴细胞和腹腔巨噬细胞作用的比较

Comparison between the Effects of Chitoolihgosacchayidean and D—Glucosamine Hydrochloride on lymphocytes and Macrophages of Mouse in Vitro

目的：观察壳寡糖和D-氨基葡萄糖盐酸盐(GAH)体外对小鼠免疫细胞作用。方法：应用MTT法观察体外壳寡糖和GAH对小鼠脾淋巴细胞细胞增殖的影响,应用巨噬细胞吞噬中性红试验观察壳寡糖和GAH对小鼠腹腔巨噬细胞吞噬功能的影响。结果：GAH浓度在200、100mg·L^(-1)时,能显著促进GonA诱导的小鼠脾细胞增殖,壳寡糖浓度为200、40mg·L^(-1)时,能增强小鼠腹腔巨噬细胞的吞噬能力。结论：结果表明壳寡糖和GHA对免疲细胞的调节作用是通过不同的途径实现的。

Objective：The purpose of this study was to assess the effects of the Chitooligosaccharidean and D--Glucosamine Hydrochloride（GAH） on immunocyte of mouse in vitro. Methods： To observe the effects of the Chitooligosaccharidean and GAH on the lymphocyte proliferation of mouse by MTT assay and the phagocytosis of macrophages by Tuluylene red pinocytic test. Results： GAH promotes lymphocyte proliferation of mouse stimulated by ConA especially when the concentration are 200mg · L^-1 and 100 mg · L^-1 in substrate and Chitooligosaccharidean markedly improves phagocytosis of macrophages of mouse when the concentration are 200mg · L^-1 and 40 nag · L^-1 in vitro. Conclusion： Chitooligosaccharidean and GAH improve of immunological function of mouse in different way.