利用FISH和DGGE对产甲烷颗粒污泥中微生物种群的研究

Study on Microbial Community in Methanogenic Granular Sludge by FISH and DGGE

利用FISH和DGGE技术对厌氧反应器内处于不同运行阶段的4个产甲烷颗粒污泥进行研究,考察其中真细菌和古细菌的种群结构,并对其中的优势古细菌进行系统发育分析.FISH结果表明,颗粒污泥中真细菌含量明显高于古细菌,真细菌主要分布在颗粒污泥外层,古细菌则主要分布在内层;DGGE结果表明,随着反应器COD负荷的增加以及运行时间的延长,真细菌种群结构相对较稳定,而古细菌种群结构则发生了较明显变化,其中占优势的古细菌种类逐渐减少;将有代表性的7个古细菌条带切胶回收并测序,结果显示,反应器运行后期占优势的菌种主要包括甲烷微粒菌(Methanocorpusculum)、甲烷杆菌(Methanobacterium)和甲烷髦毛菌(Methanosaeta)等.

Four methanogenic granules taken from an anaerobic reactor in different periods were investigated by FISH and DGGE, the eubacterial and archaeal community in these granules was researched and the phylogenetic analysis of dominant archaea was also studied. The FISH results indicated that the quantity of eubacteria was much more than archaea in the methanogenic granule and most eubacteria were located in the out layer of granule, while most archaea were located in the inner layer. The DGGE fingerprints indicated that as the organic loading rate of the reactor increased and the operating time elapsed, the eubacterial community was kept stable relatively, while the archaeal community was changed significantly, which resulted in the gradual decrease of the archaeal varieties. As seven typical bands were cut and sequenced, the results indicated that the dominant species of archaea in granule of the last period were Methanocorpusculurn, Methanobacteriurn, Methanosaeta, and etc.