CDKs在大肠癌中的表达及意义

目的探讨细胞周期依赖性蛋白激酶(CDKs)在大肠癌中的表达及临床意义。方法收集79例手术切除的大肠癌标本,同时取距癌灶5cm以外的癌旁组织及10cm以外的正常组织,采用免疫组织化学方法(链霉素抗生物素蛋白-过氧化物酶法,SP法)检测癌组织、癌旁组织及正常组织中CDKs的表达。结果79例标本癌组织中CDKs表达的阳性率均明显高于癌旁组织及正常组织,差异有显著性(P<0.01)。CDKs的表达与患者组织学类型之间均存在程度不同的统计学联系(P<0.05),此外CDK2的表达与患者年龄、肿瘤大小之间;CDC2与患者浸润深度、肿瘤大小之间;CDK4与患者年龄之间均存在统计学联系(P<0.05)。结论CDKs的过表达是大肠癌发生的早期事件,是影响大肠细胞正常细胞周期的重要途径之一。

Objective To investigate the expression of CDKs surgically resected from patients with colorectal carcinoma were collected tissue were detected by Streptavidin-Peroxidase（SP） and its clinical significance in colorectal carcinoma. Methods 79 specimens Then the expression of CDKs in colorectal carcinoma , pericacinoma and normal method, Results The positive rate of CDKs＇ expression in coloreetal carcinoma was higher than that in pericacinoma and that in nomal tissue . There was significant difference of CDKs＇ expression between the coloreetal carcinoma and pericacinoma or between the colorectal carcinoma and normal tissue（ P 〈0. 01 ）. Expression of CDKs was correlated with differentiated degree （ P 〈 0. 05）, and in addition , expression of CDK2 was correlated with patient＇ s age, diameter of carcinoma （ P 〈 0. 05） ; Expression of CDC2 was correlated with diameter of carcinoma ,invasivedepth（P〈0.01） and Expression of CDK4 was correlated with patient＇ s age （P〈0.01） . Conclusion Expression of CDKs occurs in the early stage of coloreetal carcinomatous change, which may be one of the hnportant ways in which the normal cell cycle of coloreetal cell is affected.