内源ABA对兰州百合鳞茎顶芽内物质变化的调节作用

Regulating of Endogenous Abscisic Acid on Subtances Changes in Terminal Bud of Lilium davidii var. unicolor Bulbs

以兰州百合鳞茎为试材,研究2、6、10℃条件下保湿贮藏101d内顶芽中内源激素的变化,并对内源激素与碳水化合物、酚类物质、游离氨基酸等物质的关系进行逐步回归分析。结果表明低温处理过程中ABA含量呈下降趋势,GA3、IAA和ZR含量均有升高过程。ABA和GA3含量均随贮藏温度降低而减少,且贮藏温度越低,ABA含量下降越早。相关分析结果表明GA3和ABA无任何相关性。淀粉酶活性、总可溶性糖含量、PAL活性、酚类物质含量均与ABA含量极显著负相关,而与GA3含量和ZR含量呈极显著正相关关系。通径分析结果表明ABA的负效应是最关键的因素,其次是GA3的正效应,而ZR的贡献率则相对较小。精氨酸含量与ABA含量极显著正相关,初步判定内源ABA是抑制百合顶芽萌发的主要因子。

Changes in endogenous hormones in terminal bud of Lilium davidii var. unicolor bulbs during the 101 days storage at 2 ℃, 6 ℃ and 10 ℃ in moist sawdust were studied. And the regression analyses step by step were performed farther between carbohydrate, phenols, arginine and endogenous hormones. The results indicated that abscisic acid （ABA） content decreased and gibberellic acid （GA3 ）, indo!e acetic acid （IAA） and zeatinriboside （ZR） had the increase phase during storage. As the storage temperature declined, contents of ABA and GA3 decreased, furthermore, the lower temperature was, the earlier ABA content decreased. Correlation analysis revealed that there was no any correlations between GA3 and ABA level. Significantly negative correlations between ABA content and amylase activity, total soluble sugar content, phenylalanine ammonia-lyase （PAL） activity, phenols content were observed. But there were markedly positive correlations between GA3 or ZR level and above substances. Results of path analysis showed that the negative effect of ABA was all-important, the positive effect of GA3 in the next place and effect of ZR was little. There was remarkable positive correlation between arginine and ABA concentration. It was primarily concluded that endogenous ABA was the central inhibitor and GA3 was not the essential factor for amylase activity increase in bud.