无菌松材线虫对10年生黑松致病性的研究

Studies on the Pathogenicity of 10 Years Old Black Pine Inoculated with Aseptic Pine Wood Nematode

2001年9月,用无菌的松材线虫对10年生黑松进行接种。2002年8月,检查黑松发病情况,并对接种的松树进行线虫和细菌的再分离。试验发现接种的8株黑松11个月后仍有5株表现正常,没有出现任何症状,而从其木质部却能分离到数量较多的无菌松材线虫。结果说明无菌的松材线虫虽然能侵入松树木质部,但在不携带细菌的情况下对松树是没有致病力的。

10-year-old Black Pines were inoculated with aseptic pine wood nematodes （Bursaphelenchus xylophilus ） in September 2001, and examined in August 2002. The nematodes and the bacterium were isolated from the Black Pines which were inoculated. The results indicated that 5 individuals were healthy among 8 inoculated pine trees after 11 months. The aseptic pine wood nematodes could be isolated from the xylem of healthy Black Pines. The results showed that aseptic pine wood nematodes could not lead pine trees to wilt though the nematodes could parasize inside the xylem of pine trees.