胃粘膜血流量对大鼠胃粘膜适应性细胞保护作用的影响

EFFECTS OF GASTRIC MUCOSAL BLOOD FLOW (GMBF)ON THE ROLE OF ADAPTIVE CYTOPROTECTION OF RAT GASTRIC MUCOSA

采用氢气清除法测定胃粘膜血流量（ｇａｓｔｒｉｃｍｕｃｏｓａｌｂｌｏｏｄｆｌｏｗ，ＧＭＢＦ），观察大鼠胃内灌注低浓度盐酸酒精（０．１５ｍｏｌ／ＬＨＣｌ＋１５％ＥｔＯＨ）后灌注高浓度盐酸酒精（０．６ｍｏｌ／ＬＨＣｌ＋１５％Ｅｔｏｌｌ）对ＧＭＢＦ和胃粘膜损害的影响，以及ＧＭＢＦ在适应性细胞保护中的作用。结果如下：（１）先用低浓度盐酸酒精作为弱刺激灌注随之以高浓度盐酸酒精作为强刺激灌注，引起胃粘膜适应性保护现象，它表现为：大体损伤和损伤深度分别比单独强刺激组减少４７．０９％和４４．５７％（Ｐ＜０．０１，Ｐ＜０．０５）；与此同时，ＧＭＢＦ亦出现适应性增强反应，即比单独强刺激组增加２８．０２％（Ｐ＜０．０５）；（２）近胃动脉灌注血管加压素后，ＧＭＢＦ的适应性增强反应被阻断，对胃粘膜的适应性保护作用也消失；（３）静脉注射消炎痛后，ＧＭＢＦ的适应性增强反应被阻断，对胃粘膜的适应性细胞保护作用消失，粘膜损伤程度加重。上述结果表明：低浓度盐酸酒精作为弱刺激引起的适应性细胞保护作用与ＧＭＢＦ增加反应增强有关；减弱或阻断这一增强效应可使适应性细胞保护作用消失；内源性前列腺素参与适应性细胞保护作用可能部分与ＧＭＢＦ增加有关。

By the use of hydrogen gas clearance technique, we have investigated the role ofGMBF in the adaptive cytoprotection induced by intragastric per fusion with low concentration prior to high concentration of HCl plus ethanol. The results were as follows:(1) intragastric perfusion with low concentration prior to high concentration of HClplus ethanol led to an adaptive cytoprotection, i. e., the gross and the deep damage weredecreased by 47. 09 % and 44. 57% respectively, as compared with those caused byhigh concentration of HCl plus ethanol alone; correspondingly, GMBF also showed anadaptive hyperemic response, i. e., GMBF was increased by 28. 02% as comparedwith that due to high concentration alone; (2) close arterial infusion of vasopressinblocked the adaptive hyperemic response and abolished the adaptive cytoprotection; (3)intravenous indomethacin reduced the hasal GMBF, and abolished both the adaptive hyperemic response and cytoprotection; furthermore, the gross and deep damage were aggravated compared with that caused by high concentration alone. The results showedthat the adaptive hyperemic response of gastric mucosa was involved in the adaptive cytoprotection and suggested that the adaptive cytoprotection of endogenous prostaglandinmight be partially related to the increase of GMBF.