HPLC法同时测定鸡肝散药材中3种黄酮苷元的含量

HPLC simultaneous determination of three flavonoid aglycones in Elsholtzia blanda Benth.

目的:建立一种用 HPLC 法同时测定鸡肝散药材中3种黄酮苷元含量的方法。方法:以木犀草素、芹菜素、5-羟基-6,7-二甲氧基黄酮为对照品,Agilent Zorbax SB-C_(18)(4.6mm×250mm,5μm)为色谱柱,甲醇-0.2%磷酸溶液(70:30,v/v)为流动相,检测波长350nm,流速1.0mL·min^(-1),柱温30℃。结果:木犀草素、芹菜素、5-羟基-6,7-二甲氧基黄酮3种成分浓度分别在8.54～85.4,1.2～12,9.2～92μg·mL^(-1)范围内有良好的线性关系,相关系数均为0.9999;平均加样回收率(n=9)分别为96.8%,98.0%,100.5%。结论:本法操作简便、准确、灵敏度高,可为鸡肝散药材质量标准的制定提供科学依据。

Objective：To develop a new method for simuhaneous determination of three flavonoid aglycones in Elsholtzia blanda Benth. by HPLC. Methods ： Luteolin, apigenin ,5 - hydroxy - 6,7 - dimethoxyflavone were used as reference substances,Agilent Zorbax SB - C18 （4.6 mm × 250 mm, 5 μm） was used as analytical column, with methanol -0.2% phosphoric acid solution （70： 30,v/v） as mobile phase, the detection wavelength was at 350 nm, the flow rate was 1.0 mL · min^-1 and the column temperature was 30 ℃. Results：The calibration curves of luteolin, apigenin ,5 - hydroxy - 6,7 - dimethoxyflavone were linear within the range of 8.54 - 85.4,1.2 - 12,9. 2 - 92 μg · mL^-1 ,the correlation coefficient were 0. 9999,0. 9999,0. 9999, and their average recoveries （ n = 9） were 96. 8% ,98.0% , 100. 5 % , respectively. Conclusion：The method is simple, accurate and sensitive,and can be used for quality control of E. blanda.