摘要
目的:建立复方天山堇菜颗粒中盐酸罂粟碱的含量测定方法。方法:采用 HPLC 法,色谱柱为岛津 VP-ODS 柱(150mm×4.6min,5μm),甲醇-缓冲溶液(醋酸铵0.35%,三乙胺0.03%)(60:40)为流动相,流速为1mL·min^(-1),检测波长为240nm。结果:盐酸罂粟碱的线性范围为0.13-0.64μg·mL^(-1),平均加样回收率为98.1%。结论:所建立的方法简便、可行,重复性好。
Objective : To establish a method for determination of papaverine hydrochloride in the compound Tians- hanjincai granules. Methods : HPLC method was developed for the determination of papaverinc hydrochloride in the compound Tianshanjincai granules on a column of VP - ODS ( 150 mm × 4. 6 mm, 5 μm) , methanol - buffer solution ( ammonium acetate 0. 35 %, triethylamine 0. 03 % ) (60: 40, v/v) as a mobile phase, flow rate 1 mL · min^ -1 and detection wavelength at 240 nm. Results:The range of linearity was 0. 13 -0. 64 μg · mL^-1, the average recovery was 98.1%. Conclusion:The established methods is simple, feasilble and reproducible.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第10期1486-1487,共2页
Chinese Journal of Pharmaceutical Analysis
关键词
复方天山堇菜颗粒
盐酸罂粟碱
HPLC
compound Tianshanjincai granules
papaverine hydrochloride
HPLC