运动训练和心理应激对大鼠下丘脑神经肽Y mRNA的影响

The Effect of Exercise and Phychological Stress on the Hypothalamic NPY mRNA in Rat

目的:探讨运动训练及心理应激对大鼠下丘脑神经肽Y(NPY)mRNA表达的影响。方法:将50只SD大鼠随机分为对照组、中等负荷运动组、急性心理应激组、慢性心理应激组、中等负荷运动+急性心理应激组、中等负荷运动+慢性心理应激组。实验期为10周。实验后对各组大鼠进行称重,并检测其下丘脑NPY mRNA表达。结果:(1)慢性心理应激大鼠体重显著低于对照组;急/慢性心理应激组大鼠下丘脑NPY mRNA水平均显著高于对照组。(2)中等负荷运动组大鼠体重显著高于对照组,且其下丘脑NPY mRNA水平显著高于对照组。(3)中等负荷运动+急/慢性心理应激组大鼠体重分别显著高于急/慢性心理应激组大鼠,其NPY mRNA水平与对照组相比无显著差异。结论:心理应激作为一种应激源,可能会引起大鼠神经肽分泌紊乱;中等负荷运动对提高机体抗应激能力有一定作用。

Fifty male rats were randomly divided into 6 groups：control group, moderate exercise（ swimming） group, acute psychological stress group, chronic psychological stress group, moderate exercise plus acute psychological stress group, moderate exercise plus chronic psychological stress group. Swimming exercise was lasted for 10 weeks, acute and chronic psychological stress was carried on respectively at the same time. The result showed that（ 1 ）both acute and chronic psychological stress increased hypothalamic NPY mRNA significantly. Hypothalamic NPY mRNA was significantly higher in exercise groups than that in control group;（2）The level of hypothalamic NPY mRNA in moderate exercise plus acute/chronic stress groups showed no significant difference as compared with that in control group. It was concluded that psychological stress could cause disorder of neuropeptide - excreting in rat. Exercise with moderate load could effectively reduce the psychological stress and enhance the anti - stress ability.