α-硫辛酸对高糖诱导的系膜细胞核因子-κB活性的抑制作用

Inhibitory effects of α-lipoic acid on activation of NF-κB induced by high glucose in rat mesenteric cells

目的探讨α-硫辛酸对高糖诱导的大鼠系膜细胞增殖及核因子-κB（NF-κB）活性的影响。方法在体外条件下采用正常糖（NG）、高糖（HG）及HG＋不同浓度α-硫辛酸处理大鼠系膜细胞；噻唑蓝法（MTT）测定系膜细胞增殖；采用凝胶电泳迁移率改变法检测NF-κB的活性。结果50～300μmol／L的α-硫辛酸可抑制系膜细胞增殖。HG刺激系膜细胞1h可引起NF-κB活性增强2.2倍（P〈0．01），高糖诱导的NF-κB的活化可被α-硫辛酸（200μmol／L）所抑制（0.6±0.3 vs 2．2±0．2，P〈0.01）。结论一定浓度的α-硫辛酸可抑制高糖诱导的系膜细胞增殖，并可降低NF-κB的DNA结合活性。

Objective To investigate the effects of α-lipoic acid on the proliferation and activation of NF-κB induced by high glucose （HG） in rat mesenteric cells （MCs）. Methods The rat mesenteric cells were cultured in the medium with normal glucose （5.6mmol/L, NG）, high glucose （25mmol/L, HG）, HG＋100 μmol/L α-lipoic acid, or HG＋200μmol/L α-lipoic acid and HG＋PDTC （a NF-κB inhibitor）. Activation of nuclear factor-κB （NF-κB） of rat mesenteric cells were measured by electrophoretic mobility shift assay （EMSA）. The cell proliferation was assessed by MTT. Results α-lipoic acid （50～300μmol/L） can inhibit the proliferation of MCs. The NF-κB binding activity was 2.2-fold higher in MCs exposed to HG compared to NG （P〈0.01）. When the MCs were cultured in the presence of α-lipoic acid （200μmol/L） for 1h, there was a highly significant reduction in NF-κB binding activity （0.6±0.3 vs 2.2±0.2, P〈0.01）. Conclusion It is suggested that α-lipoic acid may inhibit high glucose-induced NF-κB activation in mesenteric cells. These findings may provide an experimental evidence for further evaluating the possible protective effect of α-lipoic acid against diabetic nephropathy.