caspase-3在纳米磁药物靶向治疗胆管移植瘤组织中的表达

Expression of Caspase-3 in Targeted Therapy with Magnetic Nanoparticle in Cholangiocarcinoma Xenograft of Nude Mice

目的探讨纳米磁药物靶向治疗胆管移植瘤组织中凋亡相关基因caspase-3的表达。方法建立荷人胆管癌裸鼠模型,随机分为空白对照组、纳米磁药物组(药物浓度为250mg/kg)、纳米磁药物靶向A组(药物浓度为150mg/kg)和纳米磁药物靶向B组(药物浓度为250mg/kg);取治疗后第21d的各组肿瘤组织用免疫组化及RT-PCR法测量其中caspase-3的蛋白及mRNA表达量并进行分析。结果纳米磁药物靶向B组中caspase-3的表达量最高,其次依次为纳米磁药物靶向A组、纳米磁药物组,空白对照组几乎没有caspase-3蛋白及mRNA的表达,且各组间差异均有统计学意义(P<0.05)。结论磁靶向治疗肿瘤后能引起更多凋亡相关基因caspase-3表达,并且随纳米磁药物剂量增加,caspase-3表达增多。

Objective To investigate the expression of caspase 3 in xenograft that was treated with targeted therapy with magnetic nanoparticles in nude mice. Methods QBC939 cell lines were injected into nude mice subcutaneously to establish the model of human cholangiocarcinoma xenograft. After two weeks of tumor inoculation, the animal models were divided randomly into 4 groups： group A received placebo （sodium chloride）, group B were treated with magnetic nanoparticles （250 mg/kg）, group C were treated with magnetic nanoparticles （150 mg/kg） combined with inner-stent, group D with magnetic nanoparticles （250 mg /kg） combined with inner-stent （the innerstent was used to generate the magnetic targeting effect）. The 21th day after treatment, expression of caspase 3 in tumor cells of each groups were measured with histochemical method and RT-PCR. Results The quantity of caspase-3 in tumor cells that were treated with magnetic nanoparticles （250 mg/kg） combined with innerstent was the most （P〈0.05）, and the quantity of caspase-3 in cells of group C was significantly more than that of the other two groups （P〈0. 05）. While the quantity of caspase-3 in group B was more than that of the control group （P〈0.05）. Conclusion The use of magnetic nanoparticles combined with inner-stent may increase the expression of caspase-3, and the expression is dose-dependent with magnetic nanoparticles.