组织因子mRNA在肝癌患者组织中表达升高的意义

Significance of Tissue Factor mRNA Over-Expression in Hepatocellular Carcinoma

目的检测组织因子(TF)在肝癌组织中的表达并探讨其临床意义。方法采用RT-PCR方法检测肝癌、癌旁及对照组肝组织(各27例)中TF mRNA的表达,并结合临床病理资料进行分析。结果TF在肝癌组织中的表达阳性率及相对表达强度分别为62.96%(17/27)和0.567±0.268,均较癌旁组织的33.33%(9/27)和0.469±0.184及对照组肝组织的29.63%(8/27)和0.353±0.121高(P<0.05),其相对表达强度与肿瘤大小、肝内和肝外转移及门静脉癌栓有关(P<0.05),而与患者性别、AFP、HBsAg、是否合并肝硬变、肿瘤分化程度、癌灶数目、包膜浸润及淋巴转移均无关(P>0.05)。结论TF在肝癌组织中的表达升高与部分侵袭转移指标有关,提示其可能参与了肝细胞癌的发生及侵袭、转移。

Objective To detect the tissue factor （TF） mRNA expression in hepatocellular carcinoma and to elucidate its significance. Methods TF mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR） in 27 cases of human hepatocellular carcinoma tissue specimen with their adjacent tissues and in 27 non- tumorous process tissues. Then the relationship between mRNA expression and pathological data were analyzed. Results The expression and the relative expression intensity of TF in hepatocellular carcinoma tissues were 62.96% （17/27） and 0. 567± 0. 268 respectively, which were significantly higher than those in their adjacent tissues [33.33% （9/27）, 0. 469±0. 184] and in 27 non-tumorous process tissue [29.63% （8/27）, 0. 353±0. 121], P〈 0.05. The relative expression intensity of TF were associated with tumor size, intrahepatlc and extrahepatic metastasis and portal vein invasion, but unrelated to gender, AFP level, differentiation, HBsAg, cirrhosis, number of tumor lesions, and lymph node metastasis （P〉0. 05）. Conclusion Expression of TF mRNA were significantly higher in hepatocellular carcinoma and in the invasive and metastatic tissue, which indicated that TF may play an important role in carcinogenesis, invasion and metastasis of hepatocellular carcinoma.