乳腺癌患者血清和肿瘤组织VEGF表达与临床预后的关系

Correlation Between Expression of Vascular Endothelial Growth Factor of Serum and Tumor Tissues and Clinical Prognosis in Patients with Breast Cancer

目的探讨乳腺癌患者血清和肿瘤组织中血管内皮细胞生长因子(VEGF)的表达以及与临床预后的关系。方法以44例乳腺癌患者、13例乳腺良性疾病患者和40例正常人为研究对象,分别采用酶联免疫吸附法(ELISA)检测其血清VEGF水平,采用免疫组化LSAB法检测其组织中VEGF、雌激素受体(ER)和原癌基因C-erbB-2蛋白的表达,并分析其与临床预后因素如淋巴结转移情况及临床分期的关系。结果乳腺癌组血清和组织中VEGF表达水平(113.88pg/ml,20723.99)均明显高于乳腺良性疾病组(55.79pg/ml,3594.74),P<0.001,而乳腺良性疾病组与正常对照组(41.30pg/ml,-)比较差异无统计学意义(P>0.05);且血清和组织中VEGF的表达呈正相关(r=0.48,P<0.01)。有淋巴结转移者血清和组织中VEGF的表达水平(129.60pg/ml,28506.82)明显高于无淋巴结转移者(80.80pg/ml,14656.73),P<0.01;血清和组织中VEGF的表达与肿瘤的临床分期有关(P<0.01),但与患者年龄和肿瘤大小无关(P>0.05)。乳腺癌患者血清和组织中VEGF表达水平与组织中ER表达呈负相关(r=-0.45,P<0.05),与C-erbB-2表达呈正相关(r=0.48,P<0.01)。结论乳腺癌患者血清和肿瘤组织中VEGF表达呈正相关,且与其临床预后有关,可作为乳腺癌患者预后判断的重要参考指标之一。

Objective To investigate the correlation between expression of vascular endothelial growth factor （VEGF） of serum and tumor tissues and the clinical prognosis in patients with breast cancer. Methods The expressions of VEGF level of serum and tumor tissues in 44 patients with invasive duct breast cancer, 13 with benign breast diseases and 40 healthy controls. Serum VEGF level was measured by ELISA method. The protein expression of tissue VEGF, ER and C-erbB-2 were evaluated by immunohistochemistry LSAB method. Results Serum VEGF level and tissue VEGF expression in breast cancer were higher than those in benign breast diseases （P〈 0. 001）, and there was no significance in benign breast diseases and healthy controls （P〉0.05）. VEGF expression was correlated with lymph node metastasis （P〈0.01）, ER and C-erbB-2 expression （P〈0.05, P〈0.01） and clinical stage （P〈0. 01）. There were no statistical correlation between VEGF expression and age, tumor size （P〉 0.05）. Conclusion There is positively correlation between serum VEGF level and tissue VEGF expression, and between VEGF expression and clinic prognosis. Serum VEGF level may be one of important index of prognosis estimation in patients with breast cancer.