辣根过氧化物酶标记不同表型鼠疫F1单克隆抗体的效果观察

Observation on effect of four plague F1 antibodies labeled with horseradish peroxidase using improved method of NaIO_4

目的观察用辣根过氧化物酶(HRP)标记4种鼠疫F1抗体的效果。方法使用过碘酸钠改良法制备酶结合物,酶标抗体的效价和最适工作浓度用双抗体夹心法测定。结果3株鼠疫F1单克隆抗体中B12标记成功;鼠疫γ球蛋白标记效果较鼠疫F1单克隆抗体B12差。结论过碘酸钠改良法制备辣根过氧化物酶标记鼠疫F1单克隆抗体B12效果优于另外3种鼠疫F1抗体。

Objective To observe the effect of four plague F1 antibodies against F1 antigen labeled with horseradish peroxidase （HRP）. Methods HRP-labeled anti-F1 antibody was prepared using the improved method of NalO4. The titre and the optimal working dilution of labeled-F1 antibody were determined by double-antibody sandwich ELISA method. Results Among three HRP-labeled plague F1 monoclonal antibodies （McAb）, B12 F1 McAb was successfully labeled with HRP. The effect of HRP-labeled plague B12 F1 McAb was better than that of HRP-labeled plague γ IgG. Conclusions The effect of HRP-labeled plague B12 F1 McAb using the improved method of NalO4 is better than another 3 HRP-labeled plague F1 antibodies.