摘要
目的观察脑红蛋白(NGB)在成年大鼠脊髓中的分布。方法制备成年大鼠脊髓的冰冻切片,用免疫组织化学染色方法研究NGB蛋白在成年大鼠脊髓内的分布及细胞定位。结果在脊髓的颈、胸、腰段,NGB免疫反应阳性细胞主要分布于脊髓灰质中,其范围包括灰质脊髓前角、中间带和后角。NGB免疫反应阳性物质广泛存在于脊髓灰质神经元的胞浆中。结论NGB蛋白在大鼠脊髓灰质有广泛的表达,提示NGB在脊髓功能活动中可能起重要作用。
Objective In order to explore the localization of NGB protein in spinal cord of adult rat. Methods Spinal cord of adult rat was used to make frozen section. The localization of NGB protein in the spinal cord of adult rat was examined by immunohistochemistry method. Results NGB protein immunoreactive cells were mainly distributed in the gray matter of spinal cord, including in the ventral horn, intermediate zone and dorsal horn. NGB-immunoreactive product located in the plasm of neurons. Conclusion The results indicated that NGB widely expressed in the neurons of spinal cord, suggested that NGB might play an important role in the physiological function of the spinal cord.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第5期492-494,共3页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30400465
30571903)
关键词
脑红蛋白
脊髓
免疫组织化学
大鼠
Neuroglobin
Spinal cord
Immunohistochemistry
Rat