摘要
目的观察水通道蛋白-2(AQP-2)和水通道蛋白-4(AQP-4)在小鼠肾脏发育过程中的表达位置,探讨其与肾脏发生发育的关系及作用。方法免疫组织化学SABC法染色和体视学半定量检测AQP-2、AQP-4的表达。结果AQP-2在胚龄17d的胎鼠可以明确检测到,其表达部位为集合管主细胞的管腔膜和胞浆内。体视学测量发现,从胚龄17d开始表达到生后1d的小鼠其管腔膜的面密度值逐渐增加,此后面密度值基本上没有变化。AQP-4在胚龄14d的胎鼠可见到微弱的表达,随着胚龄的增长其表达逐渐增加,到生后1d达到最大水平,以后随着日龄的增加表达量无明显变化,并且在每一个时期的表达量都远远小于AQP-2的表达量。AQP-4的表达部位为集合管的侧基底细胞膜。结论推测AQP-2对小鼠出生后肾脏水平衡的调节作用比较重要,而AQP-4对出生前水平衡的调节作用比较重要。
Objective To observe the sites and relations of AQP-2 and AQP-4 in kidney during development and maturation, and discuss the roles of them. Methods Immunohistochemistry combined with stereological methods were used to observe and measure the expression of AQP-2 and AQP-4. Results AQP-2 was apparently noticed at the apical side and cytoplasm of principle cells in the collecting duets. The expression of AQP-2 at the apical side increased from 17-fetuses to 1-day neonatal by stereology, but it hardly changed after 1-day neonatal. The expression of AQP：4 was faint at 14-day embryo and enhanced with age to reach a plateau at birth, and was not changed remarkably thereafter. The expression of AQP-4 was less than AQP-2 in every stage of kidney development. It was localized at the basolateral membrane of ureteric buds or collecting ducts. Conclusion We presume that AQPs are very important for balance of water during development and maturation in mice kidney, while AQP-4 is before birth, AQP-2 is after birth.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第5期588-591,共4页
Acta Anatomica Sinica
基金
辽宁省教育厅基金项目资助(05L134)
