Dil散射标记神经元及神经胶质细胞技术介绍

INTRODUCTION OF DiI DIOLISTIC ASSAY TO LABEL THE NERVOUS CELL AND GLIA

目的对标记神经元和神经胶质的DiI散射法(DiIdiolisticassay)进行改进,使操作更简捷,效果更为理想。方法用C57/B6J小鼠,对固定或培养的脑片内神经元与神经胶质细胞进行DiI散射法标记。结果该方法可以显示中枢神经系统内神经元及神经胶质细胞的细微结构,其中包括树突小棘。结论Dil散射标记法标记细胞的细微结构效果得到改善,可用于对树突棘分析,特别是可以直接在培养的脑片上对神经元与神经胶质进行活体标记。

Objective The diolistic assay has been modified to make it simpler and more efficient in labeling neurons and gila. Methods CNS neurons and glial cells were labeled with DiI diolistic assay in fixed tissue and living brain slices of C57/B6J mice. Results The method allowed the visualization of the fine structure of neurons and glia including synaptic structures such as dendritic spines. Conclusion With the method, the labeling efficacy of cell＇ s fine structure is improved, making it preferable for the analysis of dendritic spine. In addition, the ability to label the living neuron and glia will extend its application vastly.