多囊卵巢综合征患者在控制性超排卵中的内分泌变化

Endocrine Status of Patients with Polycystic Ovarian Syndrome Undergoing Controlled Ovarian Hyperstimulation

目的:探讨抑制素B(Inhibin B)和胰岛素样生长因子Ⅱ(IGF-Ⅱ)与多囊卵巢综合征(PCOS)发病机制之间的关系。方法:采用酶联免疫吸附试验(ELISA)测定20例PCOS妇女和10例输卵管性因素不孕妇女控制性超排卵(COH)用药前基础血清,用药过程中(注射HCG前)血清和取卵日卵泡液(FF)中的Inhibin B和IGF-Ⅱ水平,同时采用放射免疫分析法(RIA)测定其中其他性激素水平。结果:用药后PCOS组血清Inhibin B值和IGF-Ⅱ值高于对照组(P>0.05);PCOS组卵泡液中Inhibin B和IGF-Ⅱ显著高于对照组(P<0.01);PCOS组卵泡液中IGF-Ⅱ与Inhibin B呈显著正相关(r=0.56,P<0.01)。结论:Inhibin B与IGF-Ⅱ作为重要的卵巢内局部自-旁分泌调节因子,可能参与了PCOS的发病过程。

Objective： To investigate the significance of inhibin B and insulin-like growth factor Ⅱ （IGF- Ⅱ ）in the pathogenisis of polycystic ovarian syndrome （PCOS）. Methods： The levels of inhibin B, IGF-Ⅱ and other endocrine factors in the serum and follicular fluid （FF） were assayed by ELISA and RIA before and after controlled ovarian hyperstimulation （COH）. Results： After COH, the serum levels of inhibin B （P〈0.05） and IGF-Ⅱ （P〉0.05）were higher in the PCOS group; the FF levels of inhibin B （P〈0.01） and IGF-Ⅱ （P〈0.01）of the PCOS group were obviously higher than in the control; a positive correlation was found between the FF levels of inhibin B and IGF- Ⅱ in PCOS group（r=0.56, P〈0.01）. Conclusion： The serum and FF levels of inhibin B and IGF- Ⅱ of PCOS patients undergoing COH were obviously different from the controls, which confirmed the role of inhibin B as well as IGF-Ⅱ in the pathogenisis of PCOS.