促肾上腺皮质激素释放激素受体1基因多态性与抑郁症的相关性研究

Association Study of the CRHR1 Gene and Major Depression

目的:探讨抑郁症与促肾上腺皮质激素释放激素受体1(CRHR1)基因rs242939多态性的关系。方法:采用基于Taqman-MGB的荧光定量PCR方法,检测206例抑郁症患者以及195例正常对照者的CRHR1基因rs242939位点单核苷酸多态性,分析该等位基因、基因型频率是否与抑郁症关联。结果:在正常对照组的rs242939位点的A等位基因和基因型纯合子AA的频率较抑郁症患者组高,具有显著性差异,提示rs242939位点的多态性与抑郁症的发病相关联,纯合子AA基因型对于抑郁症的发病可能起到一定的保护作用。将抑郁症分为复发性和单次发作进行分层分析,显示二组在CRHR1基因的单个SNPs的等位基因和基因型分布没有统计学上的差异。结论:CRHR1基因与抑郁症相关联。

Objective： To explore the association between the major depression and CRHR1 gene in Chinese Han people, and to provide theoretic evidence for clarifying the etiology of major depression. Methods： By using a gene-based association analysis of single-nucleotide polymorphisms （SNPs）, rs242939 SNPs were identified in CRHR1 gene and genotyped in the diagnosed samples of 206 unrelated patients with major depression and 195 matched health control subjects. Results： A-allele （P = 0.0008） and AA-genotype （P = 0.0002） was associated with rs242939, and rs242939 were significantly over-represented in controls compared to major depression patients. Conclusion ： These results support that the CRHR1 gene is likely to be involved in the genetic vulnerability for major depression.