甲状腺相关眼病眼眶成纤维细胞CD90表达的研究

The Study of CD90 Expressing in Orbital Fibroblasts of Thyroid Associated Ophthalmopathy

目的检测培养的不同来源眼眶成纤维细胞(OF)是否存在表达CD90抗原的差异,为研究眼眶成纤维细胞潜在的功能异质性奠定基础。方法培养甲状腺相关眼病(TAO)患者和正常对照眼眶成纤维细胞,流式细胞术检测CD90的表达。结果正常人、TAO患者的眼外肌、眼眶脂肪/结缔组织来源的成纤维细胞均有部分细胞表达CD90。正常人/眼外肌来源OF、TAO患者/眼外肌来源OF、正常人/眼眶脂肪来源OF、TAO患者/眼眶脂肪来源OF的CD90+细胞的比例分别为82.95%±6.49%,80.83%±7.14%,64.55%±4.45%,59.20%±11.19%。眼外肌来源的成纤维细胞中CD90+的细胞比例高于眼眶脂肪/结缔组织来源的成纤维细胞(P<0.05),而正常人与患者之间差异没有统计学意义(P>0.05)。结论正常人和TAO患者OF表达CD90抗原不一致,且与解剖部位有关。

Objective To investigate whether there is heterogeneous antigen-specific CD90 expressing on the surface of cultured orbital fibroblasts, and comparatively to study the distribution and proportion of CD90 positive cells in thyroid associated ophthalmopathy （TAO） and normal orbital tissue. Methods Human orbital fibroblasts were cultivated for normal control and TAO. Indirect immunofluoreseenee and flow eytometrie analysis were applied to detect the levels of CD90 expression. Results The CD90 antigen expressed in some of orbital fibroblasts sourced wherever from normal/TAO extraoeular muscles or normal/TAO connective/adipose tissue, in which the CD90^＋ cell occupation was 82.95%±6.49%,80. 83%±7.14%,64. 55%±4.45%,59.20%±11.19% respectively. There were more CD90^＋ fibroblasts in extraoeular muscles than in connective/adipose tissue （P〈 0. 05）, but no significant difference between normal control and TAO tissue （P〈0. 05）. Conclusions Orbital fibroblasts can be separated into CD90^＋ and CD90^- subsets with respect to surface CD90 expression. The ratio of CD90^＋ and CD90^- cells depends on their site and distribution in the orbit.