A-FABP在不同鸡种中遗传多态性分析

Analysis on Genetic Variation of Adipocyte Fatty Acid Binding Protein Gene in Different Chicken Breeds

利用PCR-RFLP技术和DNA测序检测了尤溪麻鸡、鹿苑鸡、隐性白羽鸡3个鸡种共计96只鸡的脂肪细胞型脂肪酸结合蛋白(A-FABP)第85位点和第1 805位点的遗传变异。结果表明:1)在这2个位点上,3个鸡种均存在变异,外来品种隐性白羽鸡以杂合子Cc居多;地方鸡种以cc居多,3个鸡种均含有较高频率的等位基因c,其中以尤溪麻鸡最高,而以隐性白羽鸡最低。2)A-FABP第85位点不影响A-FABP的氨基酸序列;而第1 805位点的遗传多态性影响氨基酸序列的变化:由脯氨酸变为丝氨酸,说明该多态性可能通过A-FABP基因的表达水平从而影响鸡的肉质。本试验为进一步分析A-FABP基因的遗传变异与肌内脂肪含量的关系及该分子标记在育种计划中的应用奠定基础。

The genetic variation of Adipocyte fatty acid binding protein gene on the sequence sites of 85 and 1 805 in 96 chicken including Youxi Partridge, Luyuan and Recessive White-feather chicken were detected by PCR-RFLP and DNA sequence analysis. The results showed as follows： 1）All these chicken breeds had genetic variation in A-FABP gene on sites 85 and 1 805. Recessive White-feather chicken presented mostly Cc genotype while other two breeds presented mostly cc genotype. The allele c had high frequency in three chicken breeds and in Youxi partridge chicken was the highest and Recessive White-feather chicken was the lowest. 2）The genetic variation on 85 site had no effect on amino acid sequence of A-FABP and 1 805 site had effect on amino acid sequence of A-FABP. Pro→Ser which suggested that the chicken quality may have relation to A-FABP＇s expression possibly controlled by genetic variation. The research built the base for further analysis on relation between genetic variation of A- FABP and the IMF quantity, and molecular marker＇s application in breeding.