酒精性肝病大鼠肝窦病理改变的形态学观察

Morphological observation on pathological changes of hepatic sinusoid in rats with alcoholic hepatopathy

目的研究酒精性肝病大鼠肝窦病理改变的形态学特点,探寻与肝络病相关的形态学基础。方法以“白酒、植物油、吡唑(1 000∶250∶3)”混合液灌胃制备大鼠酒精性肝病模型,造模12周后取材,肝组织石蜡切片Mallory染色,Ⅳ型胶原(ColIV)及层粘连蛋白(LN)免疫组织化学染色,肝组织超薄切片、透射电镜观察。结果与正常对照组比较,模型组大鼠肝组织内胶原含量显著增多(P<0.01);肝组织中Ⅳ型胶原及LN阳性反应颗粒面密度显著增高(P<0.01,P<0.05);肝窦内皮细胞窗孔消失,基膜连续并增厚,狄氏间隙胶原纤维增多。结论酒精性肝病大鼠肝窦主要病理改变为肝窦毛细血管化和窦周纤维增生,可以提示肝窦毛细血管化和窦周纤维化是“肝络病”的物质基础。

Objective To study the morphological characteristics of pathological changes of hepatic sinusoid in rats with alcoholic hepatopathy, and to investigate the relationships between these changes and ＂liver collateral disease＂. Methods The rat model of alcoholic hepatopathy was established by using a mixture solution of white spirit,vegetable oil and pyrazole（ 1 000： 250： 3）. After 12 weeks the liver tissue was taken. The olefin section of liver tissue was stained with Mallory method. Collagen Ⅳ （ Col Ⅳ） and LN were stained with immunohistochemistry. Meanwhile the liver tissue was sliced into ultrathin sections and observed with the transmission electron microscope （TEM）. Results The collagen content in liver tissue was increased significantly （P 〈 0.01 ）, and the Col Ⅳ content and LN density was increased in liver tissue （P 〈0. 01, P 〈0.05） in the model group compared with those of the normal control group. The fenestra of hepatic sinusoid endothelial ceils disappeared, basement membrane continued and thickened, and Disse diastem collagen fiber increased. Conclusion The main pathological changes of hepatic sinusoid in rats with alcoholic hepatopathy are capillarization and fibrosis, which were considered as the material basis of＂ liver collateral disease＂.