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应用实时定量逆转录聚合酶链反应方法检测急性早幼粒细胞白血病患者PML-RARα基因转录本的临床意义 被引量：8

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摘要 急性早幼粒细胞白血病（APL）特征性染色体改变形成了t（15；17），涉及PML—RARα融合基因。由于全反式维甲酸、砷剂的使用，目前APL患者的生存期大大延长，但是缓解期的患者仍存在复发的危险性。我们利用实时定量RT—PCR方法检测了56例APL患者PML—RARα融合基因转录本的表达，以便动态观察这些患者的治疗效果，提高治愈率。现将结果报告如下。
作者 姚利 陈子兴 岑建农 何军 邱桥成 鲍晓晶 袁晓妮
机构地区 苏州大学附属第一医院江苏省血液研究所
出处 《中华血液学杂志》 CAS CSCD 北大核心 2006年第11期773-775,共3页 Chinese Journal of Hematology
关键词 急性早幼粒细胞 基因转录本 RARα 聚合酶链反应方法 实时定量 PML 白血病患者 临床意义
分类号 R733.7 [医药卫生—肿瘤]
  • 相关文献

参考文献8

  • 1Slack JL,Bi W,Livak KJ,et al.Pre-clinical validation of a novel,highly sensitive assay to detect PML-RAR alpha mRNA using realtime reverse-transcription polymerase chain reaction.J Mol Diagn,2001,3:141-149.
  • 2Bustin SA,Benes V,Nolan T.Quantitative real-time RT-PCR-a perspective.J Mol Endocrinol,2005,34:597-601.
  • 3Viehmann S,Teigler-Schlegel A,Bruch J,et al.Monitoring of minimal residual disease (MRD) by real-time quantitative reverse transcription PCR (RQ-RT-PCR) in childhood acute myeloid leukemia with AML1/ETO rearrangement.Leukemia,2003,17:1130-1136.
  • 4顾伟英,陈子兴,曹祥山,胡绍燕,朱江,王志林,严峰,王玮,岑建农,沈慧玲,钱军.实时定量RT-PCR检测急性白血病患者骨髓细胞WT1基因表达[J].中华血液学杂志,2004,25(12):728-731. 被引量：20
  • 5顾伟英,陈子兴,胡绍燕,朱江,王志林,严峰,王玮,岑建农,沈慧玲,钱军.WT1基因水平动态检测对白血病患者异基因骨髓移植后微小残留病监测的意义[J].中华医学杂志,2005,85(7):444-447. 被引量：19
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二级参考文献16

  • 1Ogawa H, Tamaki H, Ikegame K, et al. The usefulness of monitoring WT1 gene transcripts for the prediction and management of relapse following allogeneic stem cell transplantation in acute type leukemia. Blood, 2003, 101:1698-1704.
  • 2Cilloni D, Gottardi E, De Micheli D, et al. Quantitative assessement of WT1 expression by real time quantitative PCR may be a useful tool for monitoring minimal residual disease in acute leukemia patients. Leukemia, 2002, 16:2115-2121.
  • 3Trka J, Kalinova M, Hrusak O, et al. Real-time quantitative PCR detection of WT1 gene expression in children with AML: prognostic significance, correlation with disease status and residual disease detection by flow cytometry. Leukemia, 2002,16:1381-1389.
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  • 5Szczepanski T, Flohr T, Van der Velden VH, et al. Molecular monitoring of residual disease using antigen receptor genes in childhhood acute lymphoblastic leukemia. Best Pract Res Clin Haematol, 2002,15: 37-57.
  • 6Crimwade D. The significance of minimal residual disease in pationts with t(15;17). Best Pract Res Clin Haematol, 2002,15: 137-158.?A
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  • 8Menssen HD, Siehl JM, Thiel E. Wilms tumor gene (WT1) expression as a panleukemic marker. Int J Hematol, 2002,76:103-109.
  • 9Hosen N, Sonoda Y, Oji Y,et al. Very low frequencies of human normal CD34+ haematopoietic progenitor cells express the Wilms′ tumour gene WT1 at levels similar to those in leukaemia cells. Br J Haematol, 2002, 116:409-420.
  • 10Rosenfeld C, Cheever MA, Gaiger A. WT1 in acute leukemia, chronic myelogenous leukemia and myelodysplastic syndrome: therapeutic potential of WT1 targeted therapies. Leukemia, 2003, 17: 1301-1312.

共引文献32

同被引文献72

引证文献8

二级引证文献25

中华血液学杂志
2006年 第11期

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