摘要
目的:观察阿司匹林(ASA)对大鼠局灶性脑缺血/再灌注(CI/RP)后脑内Fas配体(Fasl)表达的影响。方法:健康雄性Wistar大鼠60只,随机分成对照(A'-E')组和ASA(A-E)组,腹腔注射ASA80mg·kg-1作为干预因素,采用改良ZeaLonga线栓法制作局灶性脑缺血2h再灌注模型,用免疫组织化学染色法观察再灌注6、24、48h和4、7d脑组织Fasl表达。结果:脑缺血2h,再灌注6h在皮质及海马区即出现Fasl表达阳性细胞,24￣48h时达高峰。ASA组Fasl的表达与对照组相比P<0.05。结论:ASA可以抑制Fasl的表达,提示ASA通过抑制外源性凋亡信号的膜传导途径抑制细胞凋亡。
Aim ： To study the effects of aspirin（ASA） on the expression of Fasl in rat MCA （middle cerebral artery）-cerebral ischemia/reperfusio（CI/RP）model. Methods： 30 rats weighting 250-300 g without stroke were divided into the aspirin groups （A-E, ip ASA 80 mg·kg^-1） and the control groups （A′-E′）. The middle cerebral artery occlusion （MCAO） was made by intraluminal vascular suture for 2 h and reperfusion in the rats. The cells counts of Fasl with immunohistochemical staining were measured, respectively, in coronal sections of brain after reperfusion （6 h, 24 h, 48 h, 4 d and 7 d）. Results： The Fasl expression was seen at 6 hours after CI/RP, and reached the peak at 24-48 h. ASA may suppress the expression of Fasl after CI/RP （P 〈 0.05）. Conclusion： ASA may suppress the expression of Fasl, and suppress apoptosis viaudeath receptor signaling pathway.
出处
《中国临床神经科学》
2006年第6期594-599,共6页
Chinese Journal of Clinical Neurosciences
基金
辽宁省科技公关项目计划资助课题(2004225003-16)
