乌司他丁对内毒素诱导的脏器损伤的保护作用

Protective effects of ulinastatin on injury of organs induced by endotoxin

目的观察乌司他丁(UTI)对内毒素诱导的脏器损伤大鼠血清和肺、肝、肾组织中细胞因子的影响及肺、肝、肾组织病理改变,探讨其对脏器的保护作用。方法36只大鼠分为正常对照组、内毒素(LPS)组、治疗组。舌下静脉注射LPS诱导脏器损伤。治疗组在给予LPS前静脉注射UTI,分别于给药后2、6 h取血,同时取肺、肝、肾,制备组织匀浆并作病理。放射免疫法测定血清及肺、肝、肾组织中TNF-α、IL-6、IL-1β、iNOS。结果治疗组血清及肺、肝、肾组织TNF-α、IL-6、IL-1β、iNOS较内毒素组明显降低(P<0.05),治疗组肺、肝、肾组织的细胞变性、坏死、渗出及炎症细胞浸润明显轻于内毒素组。结论UTI能减少内毒素诱导的脏器损伤大鼠血清及肺、肝、肾组织中TNF-α、IL-6、IL-1β、iNOS的含量,减轻炎性细胞因子介导的脏器损伤,保护脏器功能,减少MODS的发生。

Aim To study protective effects of ulinastatin on injury of organs in rats induced by endotoxin. Methods 36 Wistar rats were divided into 3 groups： control group, model group and treatment group. The model of injury of organs were induced by injecting lipopolysaccharide（LPS） through the sublingual vein into rats. Ulinastatin was injected into the sublingual vein of rats in treatment group, LPS was injected into rats in the same way after 10 minutes. At the second hour or the sixth hour after treatment, serum and lung, liver and renal samples were cellected from rats to de-termine levels of TNF-α, IL-6, IL-1β and iNOS by RIA. Pathologic changes of lung, liver and renal organ were examined. Results Levels of TNF-α, IL-6, IL- 1β, iNOS of serum and renal organs in treatment group were obviously reduced than those in model group （P 〈0. 05）. Pathologic changes in treatment group were lighter than those in model group. Conclusion The ulinastatin has protection effects and prohibits the damage of the infiammtary cytokinase on the injury of organs in rats induced by endotoxin.