高脂所致脂肪肝动物模型建立的动态研究

The dynamic research of establishing fatty liver rats induced by high-fat diet

目的 建立大鼠高脂血症及非酒精性脂肪肝模型，观察肝脏脂变程度和时间的动态关系，以找到合适的高脂血症和脂肪肝模型造模时间。方法 采用Wiser大鼠50只，饲喂高脂饲料，分别检测2～6wk大鼠血清TG、TC、HDL、LDL、AST、ALT水平，根据肝脏病理切片分析肝脏脂变情况。结果 在饲喂高脂饲料2wk后，大鼠出现高血脂症状并伴有轻度的脂肪肝，随后的几周血脂水平保持稳定上升，肝系数持续增大，肝脏组织细胞脂变程度随造模时间的延长逐渐加重，3～4wk为中度，5～6wk为重度，第6周时伴有脂肪纤维化症状。结论 经过高脂饲料的喂养，6wk内可形成不同脂变程度的脂肪肝病理模型，可用于相关药理实验。

Aim To establish the fatty liver animal model induced by high-fat diet. Observe the dynamic variety of the hepatic steatosis in the different time. Finding out the ideal animal model-making time of hyperlipidemia and nonalcoholic fatty liver. Methods 50 wistar rats were fed with high-fat diet. The serum TG, TC, HDL, LDL, AST, and ALT were detected from 2 to 6 weeks. At the same time,we analyzed the hepatic steatosis situation. Results The rats developed hyperlipidemia and slight fatty liver after two weeks. In the following weeks, the ser-um lipid level and liver index kept rising. So did the amount of steatosis cells in liver tissue. The 3 -4 weeks animal model had developed moderate fatty liver and the 5 - 6 weeks animal model had developed serious fatty liver. Liver fibrosis was detected in the 6 weeks animal model. Conclusions Feeding with high-fat diet, different phases of fatty liver had been developed in six weeks, and could been used for correlative pharmacological test.