期刊文献+

高脂所致脂肪肝动物模型建立的动态研究 被引量:24

The dynamic research of establishing fatty liver rats induced by high-fat diet
下载PDF
导出
摘要 目的 建立大鼠高脂血症及非酒精性脂肪肝模型,观察肝脏脂变程度和时间的动态关系,以找到合适的高脂血症和脂肪肝模型造模时间。方法 采用Wiser大鼠50只,饲喂高脂饲料,分别检测2~6wk大鼠血清TG、TC、HDL、LDL、AST、ALT水平,根据肝脏病理切片分析肝脏脂变情况。结果 在饲喂高脂饲料2wk后,大鼠出现高血脂症状并伴有轻度的脂肪肝,随后的几周血脂水平保持稳定上升,肝系数持续增大,肝脏组织细胞脂变程度随造模时间的延长逐渐加重,3~4wk为中度,5~6wk为重度,第6周时伴有脂肪纤维化症状。结论 经过高脂饲料的喂养,6wk内可形成不同脂变程度的脂肪肝病理模型,可用于相关药理实验。 Aim To establish the fatty liver animal model induced by high-fat diet. Observe the dynamic variety of the hepatic steatosis in the different time. Finding out the ideal animal model-making time of hyperlipidemia and nonalcoholic fatty liver. Methods 50 wistar rats were fed with high-fat diet. The serum TG, TC, HDL, LDL, AST, and ALT were detected from 2 to 6 weeks. At the same time,we analyzed the hepatic steatosis situation. Results The rats developed hyperlipidemia and slight fatty liver after two weeks. In the following weeks, the ser-um lipid level and liver index kept rising. So did the amount of steatosis cells in liver tissue. The 3 -4 weeks animal model had developed moderate fatty liver and the 5 - 6 weeks animal model had developed serious fatty liver. Liver fibrosis was detected in the 6 weeks animal model. Conclusions Feeding with high-fat diet, different phases of fatty liver had been developed in six weeks, and could been used for correlative pharmacological test.
出处 《中国药理学通报》 CAS CSCD 北大核心 2006年第11期1399-1402,共4页 Chinese Pharmacological Bulletin
基金 中国科学院知识创新工程所长创新基金项目(NoY300016)
关键词 脂肪肝 高血脂 动物模型 病理 大鼠 fatty liver hyperlipidemia animal model pathology rat
  • 相关文献

参考文献11

二级参考文献75

  • 1姚凯南.第二届全国儿童保健学术会议纪要[J].中华儿童保健杂志,1994,2(3):202-203. 被引量:3
  • 2孟庆林,胡长欣,候廷奎.盐酸纳洛酮拮抗乙醇中毒作用机理的探讨[J].中国急救医学,1996,16(3):2-4. 被引量:360
  • 3李雅君 陈秉良 等.纳洛酮治疗急性酒精中毒[J].中华内科杂志,1992,31(9):564-565.
  • 4凌树森.小鼠高胆固醇血症快速造型法的研究[J].药学通报,1985,20(1):15-15.
  • 5范建高 曾民德 等.上海市4009名机关职员脂肪肝流行病学调查[J].中华肝脏病杂志,1998,6(1):17-17.
  • 6Anne-mareie L,Nicole D,Michel C et al.Effects of an antimititoc agent(cyclophosphamide )on plasma lipoproteins.Biochemical Pharmacology,1984;33(19):2961~5
  • 7苗明三.高血脂及动脉粥样硬化模型.实验动物和动物实验技术.北京:中国中医药出版社,1997:199-202
  • 8赵水平.高胆固醇血症的病因.临床血脂学.长沙:湖南科学技术出版社,1997:81-3
  • 9Reue K,Doolittle MH.Naturally occurring mutations in mice affecting lipid transport and metabolism.J Lipid Res,1996;37(7):1387~405
  • 10Mococci P,Cherubini A,Beal MF et al.Altered mitochondrial membrance uidfl fluidity in AD brain.Neurosci Lett,1996;29(2):129~35

共引文献770

同被引文献240

引证文献24

二级引证文献141

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部