HIF-1α的表达与脑膜瘤细胞增殖的关系

The Correlation of Hypoxia-inducible Factor-1α Expressing and Proliferative Activity in Meningioma

目的：探讨缺氧诱导因子-1α的表达与脑膜瘤细胞增殖的关系。方法：采用免疫组织化学方法检测48例脑膜瘤和10例正常脑膜组织中HIF-1α的表达，并通过检测增殖细胞核抗原（PCNA）的表达来反映肿瘤细胞的增殖活性，对所得资料进行统计学分析。结果：48例脑膜瘤组织中，HIF-1α阳性表达率为79％，其中弱阳性（＋），阳性（＋＋），强阳性（＋＋＋）表达率分别为33％，31％，15％；PCNA-LI为1-5，平均为1．8±0．7；正常脑膜中无HIF-1α、PCNA表达，PCNA-LI为1，明显低于肿瘤组织，差异有显著性（P〈0．01）。HIF-1α阴性表达者，PCNA为1．2±0．6；呈弱（＋）、中（＋＋）及强阳性（＋＋＋）表达者，PCNA分别为1．9±0．7、2．7±1．1及3．6±1．3，HIF-1α阳性表达程度与PCNA-LI呈正相关（ra=0．726，P〈0．01）。结论：HIF-1α的表达与脑膜瘤细胞增殖密切相关，进一步研究HIF-1α在脑膜瘤中的表达及调控血管生成细胞增殖的机制，可能为脑膜瘤的治疗开辟新方法。

Objective： To study the relationship of HIF-1α expression and proliferative activity in meningioma. Methods： Forty-eight meningioma tissues and ten human normal meningeal tissues maters expressions were detected for HIF-1α expression by SABC immunohistochemical method and the PCNA by SP immunohistochemieal method were used to label proliferative activity. The PCNA-labeling index（PCNA-LI） of tumors were calculated. Results： The positive expression rate of HIF-1α was 79% in 48 tumor tissues, among which the weak, moderate and strong positive expression rate were 33%, 31% and 15% respectively; The mean （x^-± s） of PCNA-LI was 1.8 ± 0. 7 （ ranging from 1 to 5 ） ; otherwise the expressions of HIF-1α and PCNA in normal meningeal tissues were negative, the PCNA-LI was 1. The differences of HIF-1α and PCNA-LI between meningioma tissues and human normal meninges were significant（P 〈0. 01 ）. The PCNA-LI in the negative, weak, moderate and strong HIF-1α expression samples were 1.2 ±0. 6, 1.9 ± 0. 7, 2.7 ±1.1 and 3.6 ±1.3 respectively, PCNA-LI was positively correlated with HIF-1α expression（ra = 0. 726, P 〈 0. 01 ）. Conclusion： The expression of HIF-1α in meningioma is significantly correlated to tumors＇ proliferative activity; further studying the mechanism of the HIF-1α expression and proliferative activity would lead to new ways to treat meningiomas.