大豆铁蛋白cDNA的克隆及植物表达载体的构建

CLONING OF SOYBEAN FERRITIN GENE AND CONSTRUCTION OF ITS PLANT EXPRESSION VECTOR

以大豆为材料,利用RT-PCR法扩增得到大小约750bp的片段,将这个片段连接到克隆载体pBlueskript SK+上进行测序,结果证明,此片段就是大豆的铁蛋白基因。将此片段再正向插入到植物表达载体pBI121的CaMV35s启动子和NOS终止子之间,构建了植物表达载体pBIFe,并成功地将该载体导入根癌农杆菌EHA105。此项工作为获得表达铁蛋白的转基因植物奠定了基础。

A 750bp fragment was amplified by RT-PCR from soybean. The PCR products were cloned into pBlueskript SK＋ vector and named pFer after its sequences was confirmed. The soybean ferritin gene was subcloned into plant expression vector pBI121 and named PBI121Fer. Then the recombination plasmid were introduced into Agrobacterium EHA105. This work provides a foundation for transferring ferritin gene into plant by Agrobacterium mediated transformation.