头孢米诺钠与2,4-二硝基苯酚的荷移反应及其测定

Spectrophotometric determination of cefminox sodium based on the transfer reaction between cefminox sodium and 2,4-dinitrophenol

目的建立用荷移分光光度法测定头孢米诺钠含量的方法。方法利用头孢米诺钠与2，4-二硝基苯酚在甲醇一丙酮介质中反应，形成电荷转移络合物，采用分光光度法测定。结果荷移络合物在403nm处有最大吸收，表观摩尔吸光系数为ε=1．47×10^4L／（mol·cm），药物浓度在4～40μg／ml范围内符合比耳定律线性关系，方法平均回收率大于97．0％。结论荷移分光光度法简便、准确、灵敏，可作为头孢米诺钠制剂的含量测定方法。

Objective To establish a spectrophotometric method for the determination of cefminox sodium, based on charge-transfer reaction. Method Cefminox sodium reacted with 2, 4-dinitrophenol to form a charge-transfer complex in methanol-acetone and can be quantitatively determined by spectrophotometric method at 403 nm. Results Beer＇s law is obeyed in the range of 4-40 μg/ml of cefminox sodium. The apparent molar absorptivity of CT at 403nm is ε=1.47×10^4L/（mol·cm）. The average recovery was above 97.0%. Conclusion The method is simple, sensitive and accurate to determine cefminox sodium.