HPLC法测定阿奇霉素散剂的含量

Content determination of azithromycin powder by HPLC

目的建立HPLC法测定阿奇霉寨散剂的含量。方法采用C8柱，以0．045mol／L磷酸二氢胺溶液；乙腈（2：1）（用三乙胺调pH至7．35）为流动相，流速1ml／min．检测波长210nm，柱温40℃。结果阿奇霉紊的线性范围为50．3～1006μg／ml，平均回收率100．1％，r=1．0000，RSD=0．8％（n=9）。结论HPLC法测定结果与微生物检定法比较无显著性差异。本方法可准确、快速、简便地测定阿奇霉素散剂的含量。

Objective To establish an HPLC method for the determination of azithromycin powder. Method The chromatographic conditions were as followed： C8 column, 0. 045 mol/L ammonium phosphate monobasic solution ： acetonitrile （2 ： 1） （adjusted to pH7.35 with triethylamine） as mobile phase, temperature： 40℃, the flow rate; 1.0 ml/min, detection wavelength： 210am. Results The linear range was in the range of 50.3-1006μg/ml （r=1. 0000）, and the mean recovery （n=9） was 100.1% （RSD=0.8%）. Conclusion The method is rapid, simple and accurate