噻奈普汀对强迫游泳应激大鼠行为及血清皮质酮的影响

Effect of tianeptine on behaviour and serum corticosterone of stressed rats

目的探讨应激导致大鼠行为和血清皮质酮浓度的改变以及噻奈普汀的抗抑郁机理。方法将24只大鼠随机分为对照组、4周应激组、4周应激给水组和4周应激给药组,各应激组大鼠连续给予强迫游泳试验4周,每天持续15min。游泳后给予灌胃,4周应激给药组按50mg/kg给予噻奈普汀生理盐水溶液灌胃,4周应激给水组按相同比例给予生理盐水灌胃,采用Open-Field法评定行为,使用酶联免疫吸附测定法(ELISA)测定血清皮质酮水平。结果应激后对照组爬格数明显高于4周应激组和4周应激给水组(47.50±29.66)次,(12.67±11.38)次,P(0.05;(47.50±29.66)次,(7.67±3.01)次,P(0.05),而与4周应激给药组比较差异无显著性(P>0.05);对照组大鼠修饰次数明显高于4周应激组和4周应激给水组[(7.00±1.79)次,(2.50±2.35)次,P(0.05;(7.00±1.79)次,(3.17±3.19)次,P(0.05],而与4周应激给药组相比差异无显著性(P>0.05)。对照组血清皮质酮明显低于其他3组(P(0.01)。结论应激可以导致大鼠焦虑样行为增加和皮质酮激素水平升高,噻奈普汀可以有效减少应激所致焦虑样行为。

Objective To explore the mechanism of stress-induced changes of behaviour and serum corticosterone concentration and the antidepression mechanism of tianeptine. Methods 24 rats were randomly allocated into control（n=6） , 4 week-stress（n=6） , 4 week-stress with saline（n=6） and 4 week-stress with tianeptine（ n =6） groups, Forced Swimming Test was used to set up stress animal model and to assess the stress result by behaviour test and concentration of serum corticosterone. Results The crossing number of control group is higher than 4 week-stress group and 4 week-stress with saline after stress （47.50 ± 29.66,12.67±11.38, P 〈 0.05 ;47.50 ± 29.66,7.67±3.01, P 〈 0. 05 ）, while no difference was found compared with 4 week-stress with tianeptine group. Compared with 4 week-stress group, the number of ornament of control group is much higher （7.00 ± 1. 79,2.50±2.35, P 〈 0.05 ）, there is no difference when compared with 4 week-stress with saline group and 4 week-stress with tianeptine group. Concentration of serum corticosterone of control group is much lower than other groups（ P〈 0.01 ）. Conclusion Stress could induce elevation of anxiety like behavior and increase of serum corticosterone concentration, and tianeptine can decrease anxiety like behavior induced by stress.