ICAM-1和IGF-Ⅱ在胆管癌中的表达及与预后关系

Expression of ICAM-1 and IGF-Ⅱ in cholangiocarcinoma and correlation with prognosis

目的探讨胆管癌细胞ICAM-1和IGF-Ⅱ的表达与胆管癌转移、预后关系。方法常规石蜡切片,用鼠抗人IGF-Ⅱ、ICAM-1单克隆抗体免疫组化法,光学显微镜观察胆管癌细胞阳性染色。结果(1)胆管癌IGF-Ⅱ阳性表达率为52.9%(36/68),主要在胞浆和胞核表达;I-CAM-1阳性表达率为61.8%(42/68),主要在胞浆和胞膜表达。(2)ICAM-1和IGF-Ⅱ在分化好的胆管癌表达率低于分化差的胆管癌。(3)IGF-Ⅱ高表达,肿瘤组织中内皮细胞丰富,与血管生成密切相关。ICAM-1和IGF-Ⅱ高表达,存活时间短,易发生转移(88.9%)。结论胆管癌ICAM-1和IGF-Ⅱ共同高表达,使肿瘤细胞更易于从肿瘤组织中脱落、迁移,使胆管癌细胞易于侵袭周边组织,增加远处转移机会。

Objective To evaluate the immanohistochemical IGF- Ⅱ and ICAM- 1 overexpression and relationship with metastasis and prognosis of eholangiocarcinoma. Methods Immunohistochemical staining of ICAM - 1 and IGF -Ⅱ was performed on tissue paraffin - embedded samples of cholangioearcinoma and positive staining cells observed under a microscope. Results （1） The positive cases of ICAM- 1 and IGF- Ⅱ were 42 （61.8%） anc 36 （52.9%） in 68 cases of cholangioearcinoma respectively. The expression of ICAM- 1 on cell membrane or in cytoplasm, and IGF- Ⅱ in cytoplasm and nucleus was detectable. （2）ICAM - 1 and IGF -Ⅱ positive expression was significantly higher in poorly or un-differentiated cholangiocarcinoma than in well- differentiated. （3） Overexpression of IGF - Ⅱ and many endothelial cells in cholangiocarinoma ware closely correlated with angiogenesis. Overexpression of ICAM - 1 and IGF- Ⅱ was closely assoeiated with metastasis and survival. Conclusion IGF- Ⅱ and ICAM- 1 overexpression facilitates cholan giocarcinoma invasion and metastasis, which may play an important role in tumor progression and metastatis.