摘要
目的 探讨幽门螺杆菌(Hp)感染与白细胞介素-1基因家族中白细胞介索-1B(IL-1B)-31与IL-1B-511(IL-1B)位点单核苷酸多态性(SNP)、IL-1RN可变数目串连重复序列(VNTR)及二者交互作用与十二指肠溃疡病(DU)发病风险的关系。方法 用病例对照研究方法,调查94例对照和54例DU患者,ELISA方法检测患者IgG抗体,PCR扩增目的基因,电泳检测IL-1RN可变数目串连重复序列,限制性片段多态性分析(RFLP)检测IL-1B-31、IL-1B-511单核苷酸多态性。结果 Hp感染、IL-1B-511C/C型增加DU的患病危险,OR分别为8.26(95%CI=3.02~22.59),3.05(95%CI=1.23~7.57)。Hp感染和IL-1B-31T/T、IL-1B-511C/C、非Ⅰ型IL-1RN之间有交互作用存在,交互作用项OR分别为16.75(95%CI=3.64~77.04),41.42(95%CI=8.96~191,40),4.93(95%CI=1.35~17.99),交互作用系数(γ)分别为1.22,1.7,0.84。结论 IL-1B-31T/T、IL-1B-511C/C能增加Hp感染对十二指肠溃疡病患病的危险作用,而非Ⅰ型IL-1RN则减弱Hp的危险作用。这种交互作用的存在有助于解释Hp感染临床结局的复杂性。
Objective To elucidate relations among H. pylori infection and interleukin- 1 locus polymorphisms: IL- 1B - 31, IL- 1B - 511 and IL- 1RN and to investigate interaction of both in development of duodenal ulcers. Methods Case - control study was carried out involving 94 control subjects and 54 patients with duodenal ulcer. H. pylori states was examined with ELISA, VNTR of IL- 1RN was examined with PCR, PCR - RFLP was used to analyze SNPs of IL - 1B - 31 and IL - 1B- 511. Results Helicobacter pylori infection, carriage of IL- 1B- 511 C/C increased risk of duodenal ulcer with odds ratios of 8.26 (95% CI = 3.02 - 22.59) and 3.05 (95% CI = 1, 1.23 - 7.57) respectively. Odd ratios of interaction between H. pylori infection and IL- 1B - 31T/T, IL- 1B - 511C/C, non - I IL - 1RN were 16.75(95% CI= 3.64 - 77. 04), 41.42(95 % CI = 8.96 - 191.40), 4.93 (95 % CI = 1.35 - 17.99), and γ was 1.22, 1.7, 0.84 respectively. Conclusion H. pylori infection and IL- 1B- 31T/T, IL- 1B - 511 C/C synergistically determine susceptibility to duodenal ulcer, IL - 1RN can reduce the risk effect of H. pylori infection. The interaction is helpful to explain the complexity of H. pylori-assoelated disease.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第11期1366-1368,共3页
Chinese Journal of Public Health