幽门螺杆菌与白细胞介素-1基因交互作用

Interaction between Helicobacter pylori and interleukin-1 gene

目的 探讨幽门螺杆菌（Hp）感染与白细胞介素-1基因家族中白细胞介索-1B（IL-1B）-31与IL-1B-511（IL-1B）位点单核苷酸多态性（SNP）、IL-1RN可变数目串连重复序列（VNTR）及二者交互作用与十二指肠溃疡病（DU）发病风险的关系。方法 用病例对照研究方法，调查94例对照和54例DU患者，ELISA方法检测患者IgG抗体，PCR扩增目的基因，电泳检测IL-1RN可变数目串连重复序列，限制性片段多态性分析（RFLP）检测IL-1B-31、IL-1B-511单核苷酸多态性。结果 Hp感染、IL-1B-511C／C型增加DU的患病危险，OR分别为8．26（95％CI=3．02～22．59），3．05（95％CI=1．23～7．57）。Hp感染和IL-1B-31T／T、IL-1B-511C／C、非Ⅰ型IL-1RN之间有交互作用存在，交互作用项OR分别为16．75（95％CI=3．64～77．04），41．42（95％CI=8．96～191，40），4．93（95％CI=1．35～17．99），交互作用系数（γ）分别为1．22，1．7，0．84。结论 IL-1B-31T／T、IL-1B-511C／C能增加Hp感染对十二指肠溃疡病患病的危险作用，而非Ⅰ型IL-1RN则减弱Hp的危险作用。这种交互作用的存在有助于解释Hp感染临床结局的复杂性。

Objective To elucidate relations among H. pylori infection and interleukin- 1 locus polymorphisms： IL- 1B - 31, IL- 1B - 511 and IL- 1RN and to investigate interaction of both in development of duodenal ulcers. Methods Case - control study was carried out involving 94 control subjects and 54 patients with duodenal ulcer. H. pylori states was examined with ELISA, VNTR of IL- 1RN was examined with PCR, PCR - RFLP was used to analyze SNPs of IL - 1B - 31 and IL - 1B- 511. Results Helicobacter pylori infection, carriage of IL- 1B- 511 C/C increased risk of duodenal ulcer with odds ratios of 8.26 （95% CI = 3.02 - 22.59） and 3.05 （95% CI = 1, 1.23 - 7.57） respectively. Odd ratios of interaction between H. pylori infection and IL- 1B - 31T/T, IL- 1B - 511C/C, non - I IL - 1RN were 16.75（95% CI= 3.64 - 77. 04）, 41.42（95 % CI = 8.96 - 191.40）, 4.93 （95 % CI = 1.35 - 17.99）, and γ was 1.22, 1.7, 0.84 respectively. Conclusion H. pylori infection and IL- 1B- 31T/T, IL- 1B - 511 C/C synergistically determine susceptibility to duodenal ulcer, IL - 1RN can reduce the risk effect of H. pylori infection. The interaction is helpful to explain the complexity of H. pylori-assoelated disease.